2017年福建省监测点致泻性大肠埃希菌监测及耐药性分析

Monitoring and antibiotic resistance analysis on diarrheagenic Escherichia coli in monitoring sites,Fujian province,2017

目的了解福建省致泻性大肠埃希菌(DEC)流行特点及耐药情况,为预防与控制福建DEC腹泻提供依据。方法收集2017年福建省永安与南平市腹泻人群粪便标本共300份,经初步培养分离后利用PCR方法进行毒力基因检测,并对检出的病原菌进行血清学试验,运用K-B纸片法进行耐药性分析。结果 300份标本中检出已纯化单克隆DEC 22株,检出率为7.33%,其中EAEC 9株(40.91%),ETEC 9株(40.91%),aEPEC 4株(18.18%);检测分离到血清凝集菌株4株。药敏结果显示:DEC对氨苄西林、复方新诺明耐药率较高,分别为59.09%、36.36%;其中1株菌对6类抗菌药物均耐药;一株产ESBLs菌株。结论福建省DEC流行属种以EAEC、ETEC为主,且具有较高的耐药性。

Objective To understand the epidemiological characteristics and antibiotic resistances of diarrheagenic Escherichia coli（DEC）in Fujian province,so as to provide basis for the prevention and control of DEC diarrhea. Methods In2017,a total of 300 stool specimens were isolated from diarrhea patients in Yongan and Nanping cities,then performed using PCR methods to detect the virulence genes after the traditional isolation and identification.Antibiotic resistance was measured using Kirby-Bauer disc diffusion assay. Results A total of 22 strains of DEC were detected from 300 specimens with the detection rate of 7.33%,including 9 strains of EAEC（40.91%）,9 strains of ETEC（40.91%）,and 4 strains of aEPEC（18.18%）.There were 4 stains which can be diagnosed by DEC sera.High resistance rate of DEC was detected to ampicillin（59.09%）and cotrimoxazole（36.36%）,respectively.One strain was resistant to 6 kinds of antibiotics.One strain of ESBLs producing strain was detected. Conclusion Most of DEC are EAEC and ETEC in Fujian province.The DEC have high antibiotic resistances.