脂联素基因多态性与冠心病的关联研究

Association between ADIPOQ gene polymorphisms and coronary artery disease

目的:探讨广东汉族人群脂联素基因(ADIPOQ)单核苷酸多态性(SNP)位点与冠心病的关联性。方法:运用SNPscanTM多重SNP分型试剂盒对1 044例冠心病和1 349例健康对照进行SNP位点检测。χ~2检验和Logistic回归分析用于检测SNP位点与冠心病的关联性。结果:冠心病组rs266729的GG、GC和CC基因型频数(频率)分别为69(6.6%)、385(36.9%)和590(56.5%),对照组分别为77(5.7%)、498(36.9%)和774(57.4%);冠心病组rs16861205位点的AA、AG和GG基因型频数(频率)分别为28(2.7%)、250(23.9%)和766(73.4%),对照组分别为37(2.7%)、365(27.1%)和947(70.2%)。两位点的等位基因和基因型频率在冠心病组和对照组间差异无统计学意义(P>0.05)。Logistic回归分析显示在3种遗传模型(加性、显性和隐性)下两位点的基因型不是冠心病的危险因素(P>0.05)。结论:本研究未提示脂联素基因rs266729和rs16861205位点与广东汉族人群冠心病有关联。

Objective：To investigate the association of the single nucleotide polymorphisms located in ADIPOQ gene with coronary artery disease（CAD）susceptibility in Chinese Han population in Guangdong province.Method：A total of 1044 CAD patients and 1349 healthy individuals were genotyped using SNPscanTM multiple SNP genotyping assays,and the association between the three SNP loci and CAD was tested by Chi-square test and Logistic regression model.Result：The frequencies of GG,GC and CC of rs266729 were 69（6.6%）,385（36.9%）,and 590（56.5%）in the cases and 77（5.7%）,498（36.9%）and 774（57.4%）in controls.The frequencies of AA,AG,and GG of rs16861205 were 28（2.7%）,250（23.9%）and 766（73.4%）in the cases and 37（2.7%）,365（27.1%）,and947（70.2%）in controls.There was no statistically significant difference between the patients with CAD and the health controls in terms of allelic and genotypic frequency distribution at the two polymorphic loci（all P〉0.05）.Logistic regression analysis also did not reveal significant effect of these SNPs on CAD under three genetic models（additive,dominant and recessive）（all P〉0.05）.Conclusion：This study lack adequate evidence to support the association of ADIPOQ gene rs266729 and rs16861205 polymorphisms with CAD in Chinese Han population in Guangdong province.