蒙药泡囊草化学成分的分离与鉴定

Isolation and identification of chemical constituents from Physochlaina phsaloides

目的研究蒙药泡囊草不同溶剂提取物的化学成分。方法采用硅胶、Sephadex LH-20柱色谱法和半制备高效液相色谱法对不同溶剂提取物进行分离纯化,运用各种光谱法鉴定化合物的结构。结果与结论鉴定了10个单体化合物,分别为7-甲氧基香豆素(1)、7-羟基香豆素(2)、6,7,8-三甲氧基香豆素(3)、8-羟基-6,7-二甲氧基香豆素(4)、6,7-二羟基-8-甲氧基香豆素(5)、6-甲氧基香豆素-7-O-β-D-葡萄糖苷(6)、6,8-二甲氧基香豆素-7-O-β-D-葡萄糖苷(7)、京尼平苷(8)、当药苦苷(9)和梓苷(10)。化合物1~10均为首次从该植物中分离得到。

Ten compounds were isolated from Physochlaina phsaloides. Chromatographic techniques were used for the isolation and purification, including LH-20 column chromatography, silica gel and preparative HPLC. The structures were identified on the basis of physicochemical properties and spectroscopic analysis. Ten compounds were obtained and their structures were identified as 7-methoxycoumarin （1）, 7-hydroxy coumarin （ 2 ）, 6,7,8-trimethoxycoumarin （ 3 ）, 8-hydroxyl-6,7-dimethoxycoumarin （ 4 ）, 6,7-dihydroxyl-8- methoxycoumarin （ 5 ）, 6-methoxycoumarin-7-O-β-D-glucoside （ 6 ）, 6, 8-dimethoxycoumarin-7-O-β-D- glucoside （7）, geniposide （8）, swertiamarin （ 9 ） and catalposide （ 10 ）. Compounds 1 - 10 were isolated from this plant for the first time.