成人支气管哮喘患者急性发作期外周血IL-27表达变化及意义

Expression changes and clinical significance of interleukin-27 in adult patients with acute exacerbation of asthma

目的观察成人支气管哮喘患者急性发作期外周血白细胞介素27(IL-27)表达变化,并探讨其临床意义。方法选择支气管哮喘急性发作期患者20例作为哮喘组、体检健康者12例作为对照组。采集两组外周静脉血,提取血清及单个核细胞。用流式细胞术检测表达IL-27的树突状细胞比例;实时荧光定量PCR技术检测外周血单个核细胞IL-27亚基(p28、EBI3)及Th1、Th2细胞特异性转录因子(T-bet、GATA3)mRNA表达;酶联免疫吸附试验检测血清中IL-27、Th1细胞因子γ干扰素(IFN-γ)、Th2型细胞因子白细胞介素4(IL-4)水平,并与IL-27做相关分析。结果哮喘组外周血IL-27阳性树突状细胞比例较对照组低(P<0.05)。与对照组比较,哮喘组外周血p28、EBI3、T-bet mRNA表达及T-bet/GATA3低(P均<0.05),两组GATA3 mRNA表达比较差异无统计学意义(P>0.05)。与对照组比较,哮喘组血清IL-27、IFN-γ水平低(P均<0.05),血清IL-4水平高(P<0.05)。哮喘组血清IL-27水平与IFN-γ呈正相关(r=0.693,P<0.01),与IL-4无相关性(r=0.259,P>0.05)。结论成人支气管哮喘患者急性发作期外周血中IL-27呈低表达,其可能通过作用于Th1细胞参与哮喘的发生发展。

Objective To investigate the expression changes and clinical significance of interlukin-27（ IL-27） in the peripheral blood of adult patients with acute exacerbation of asthma. Methods Twenty adult patients with acute exacerbation of asthma were collected as the asthmatic group,while 12 healthy controls from medical examination center were selected as the control group. Heparinized venous blood was collected from the two groups,and then peripheral blood mononuclear cells（ PBMCs） were separated from the blood. The proportion of IL-27＋dendritic cells（ DCs） was evaluated by flow cytometry. The mRNA expression levels of IL-27 subunits（ p28,EBI3）,Th1 and Th2 specific transcription factors（ Tbet,GATA3） in the PBMCs were detected by quantitative real-time PCR（ q PCR）. The levels of serum IL-27,Th1 specific cytokine interferon-gamma（ IFN-γ）,Th2 specific cytokine interleukin 4（ IL-4）,and the correlations between them were examined by ELISA. Results Asthmatic patients showed down-regulated proportion of IL-27＋DCs as compared with the controls（ P〈0. 05）. Compared with the control group,the mRNA expression levels of p28,EBI3,T-bet,and the magnitude of T-bet expression versus GATA3（ T-bet/GATA3） decreased in the asthmatic group（ all P〈0. 05）,while GATA3 mRNA expression was not statistically different between the two groups（ P〈0. 05）. Compared with the control group,the levels of serum IL-27 and IFN-γ significantly decreased,while the IL-4 level increased in the asthmatic group（ all P〈0. 05）. Moreover,in the asthmatic group,the level of serum IL-27 was positively correlated with IFN-γ（ r =0. 693,P〈0. 001）,but was not related with IL-4（ r = 0. 259,P〈0. 05）. Conclusion The IL-27 is low expressed in the peripheral blood of adult patients with acute exacerbation of asthma,which suggests that IL-27 could contribute to the pathogenesis of asthma by regulating Th1 cells.