贵州地区麻风与人白细胞抗原A及人白细胞抗原B等位基因的小样本关联性研究

Analysis of association of HLA-A and HLA-B with leprosy in Guizhou

目的:研究分析人白细胞抗原(HLA)-Ⅰ类基因HLA-A、HLA-B等位基因与贵州地区麻风相关性。方法:采用序列特异性引物聚合酶链反应法(PCR-SSP)对43例麻风患者及23例健康对照组进行HLA-A、HLA-B等位基因的检测、分型,采用χ~2检验比较分析两组之间等位基因频率的差异。结果:麻风组HLA-A~*02等位基因频率低于健康对照组,差异有统计学意义(P<0.05);瘤型麻风组HLA-A~*02等位基因频率低于健康对照组,差异有统计学意义(P<0.05);瘤型麻风组HLA-B~*46等位基因频率低于健康对照组,差异有统计学意义(P<0.05)。结论:HLA-B~*46可能是瘤型麻风的保护基因;HLA-A~*02可能是麻风的保护基因。

Objective： To explore the association of HLA-A and HLA-B alleles with leprosy. Methods： Polymerase chain reac- tion-sequence specific primers（PCR-SSP） were used to genotype HLA-A and HLA-B alleles in 43 leprosy patients and 23 healthy controls. Chi-square test was used to determine the significanees of frequencies between patients and controls. Results： The allele frequencies of HLA-A＊02 were lower in leprosy patients than in healthy controls （P〈0.05）. The allele frequencies of both HLA- A＊02 and HLA-B＊46 were lower in LL leprosy patients than in healthy contrels（P〈0.05）. Conclusions： HLA-B＊46 could be pro- tective gene only for LL leprosy, while HLA-A＊02 may be protective gene for all subtypes of leprosy.