微囊藻毒素在大型潜流人工湿地中的去除效果

Removal Effect of Microcystins by a Large-scale Subsurface Flow Constructed Wetland

在优化酶联免疫吸附分析方法(ELISA)的基础上,测定了人工湿地系统中的微囊藻毒素浓度,考察了大型潜流人工湿地对北方城市河道水体中微囊藻毒素的去除效果。结果表明,优化后的ELISA法能够准确测定水体中低浓度的微囊藻毒素,准确性>76%。人工湿地进水中的微囊藻毒素浓度呈季节性变化,5月—6月浓度最高,其次是9月—11月、7月—8月,3月—4月浓度最低;人工湿地对微囊藻毒素的去除率在47.03%~87.22%之间,平均为59.25%。不同植物组合(芦苇/千屈菜/鸢尾、香蒲/千屈菜/花叶芦荻/鸢尾、芦苇/千屈菜)湿地单元对水体中微囊藻毒素的去除无显著差异,平均去除率分别为58.9%、57.5%、62.0%,低浓度微囊藻毒素的去除与潜流人工湿地的植被生长状况无显著性关系。

Based on optimized enzyme-linked （MCs） concentration and their removal efficiency from immunosorbent assay （ ELISA ）, microcystins urban water body in northern China by a large- scale subsurface flow constructed wetland system was studied. The results showed that the optimized ELISA could accurately determine the low concentration of MCs with accuracy higher than 76%. The MCs concentration in influent of the constructed wetland varied seasonally： the concentrations were the highest from May to June, the next were September to November and July to August, and concentrations were the lowest from March to April. The removal rates of MCs were between 47.03% and 87.22% with an average removal rate of 59.25%. Wetland units with different vegetation combinations （Phragmites commurtis/Lythrum salicaria/lris tectorum, Typha latifolia/Lythrum salicaria/Arundo donax var. versico- lor/ lris tectorum, and Phragmites communis/Lythrum salicaria ） did not show significant impacts on removal of MCs in water body, where the average removal rates were 58.9%, 57.5% and 62.0% spectively. There was no significant relationship between the removal of low concentration MCs and the re- the vegetation growth of the subsurface constructed wetland.