ASBR系统中磷化氢浓度与碱性磷酸酶活性的关系

Relationship between Phosphine Concentration and Alkaline Phosphatase Activity in ASBR System

以厌氧序批式反应器(ASBR)为研究对象,通过对反应器进水和出水中TP、COD和沼气中自由态磷化氢的长期监测,确定体系存在稳定的磷化氢产出。在此基础上,研究了系统在典型周期内TP、COD、磷化氢和碱性磷酸酶的变化规律,分析了磷化氢和碱性磷酸酶之间的相互关系。结果表明:TP、COD在反应过程中呈下降趋势且在5 h时实现最大去除,随后上下小幅波动;气态磷化氢(PH3)变化趋势呈"S"形;结合态磷化氢(MBP)和碱性磷酸酶活性(APA)变化趋势一致,均于3 h时骤升、4 h陡降、7 h小幅上升,然后波动;PH_3与APA没有明显的相关关系,MBP与APA呈显著正相关(R^2=0.964,P<0.01)。

The production of phosphine in anaerobic sequencing batch reactor （ASBR） was stud- ied. After the long-term monitoring of total phosphorus （ TP）, chemical oxygen demand （ COD）, and phosphine, it was determined that the system had a stable production of phosphine. The changes in TP, COD, phosphine, and alkaline phosphatase in the typical cycle were studied, and the relationship be- tween phosphine and alkaline phosphatase was analyzed. TP and COD showed a decreasing trend in the reaction process, reached a maximum removal at 5 h, and then fluctuated slightly. The change of gaseous phosphine （PH3 ） showed an ＂S＂ shape. The matrix-bound phosphine （MBP） and alkaline phosphatase activity （APA） both rose dramatically at 3 h, dropped sharply at 4 h, and then increased moderately at 7 h. No significant correlation was identified between PH3 concentrations and APA; while, MBP concen- trations showed a significant positive correlation with APA （ R2 = 0.964, P 〈 0.01 ）.