扫描电镜与显微镜观察枸杞花药败育过程比较

COMPARISON OF OBSERVATION BY SCAMING ELECTRON MICROSCOPE AND OPTICAL MICROSCOPE OF ANTHER ABORTION PROCESS OF Lycium barbarum

以宁夏中卫主栽枸杞品种‘宁杞1号’为对照,采用石蜡切片技术,用扫描电镜与显微镜系统跟踪观察枸杞‘YX-1’花药败育过程,从而比较二者在观察花药败育过程中的优劣。结果表明:在观察绒毡层形成周原质团、花母细胞分裂时显微镜优于扫描电镜,花药壁部分细胞转化成似维管束细胞、花药壁维管束细胞与花粉囊分离及花粉饱满程度时扫描电镜优于显微镜。用扫描电镜观察"YX-1"为完全不育株,与显微镜观察结果不一致。在植物育性鉴定方面,利用扫描电镜观察植物花药败育过程是一种可以利用的新途径和新方法。

By taking ＂Ningqi No. 1 ＂, the main medlar variety cultivated in Zhongwei, Ningxia as a comparison, adopting the paraf- fin sectioning technique, and using the scanning electron microscope and the microscope system to track and observe the anther abor- tion process of medlar ＂YX - 1 ＂, a comparison was made between the strengths and weaknesses of the two systems in the observation of the anther abortion process. The findings showed ： when observing the formation of the periplasmodium by the tapetum and the divi- sion of floral mother cells, the microscope is better than the scanning electron microscope, and when observing the transformation of anther wall cells into vascular - bundle - like cells, the separation of anther wall vascular bundle cells from the anther, as well as the plumpness of pollen, the scanning electron microscope is better than the microscope. ＂YX - 1＂ is a completely sterile plant according to the observation using the scanning electron microscope, which is inconsistent with the observation result of the microscope. In terms of plant fertility identification, the scanning electron microscope is a new approach and method that can be adopted when observing the anther abortion process of plants.