单分子荧光共振能量转移用于生物大分子构象动态变化过程研究进展

Progresses of Single Molecular Fluorescence Resonance Energy Transfer in Studying Biomacromolecule Dynamic Process

生物大分子参与生命活动的各个过程,在单分子水平上实时观测和分析生物大分子自身的结构动态以及生物大分子相互作用的动态过程,对于深入理解生物大分子的作用机制具有重要意义。自提出以来,单分子荧光共振能量转移技术逐渐展现出其在研究生物大分子构象变化和相互作用过程等方面的巨大潜力,一系列新的作用机理陆续被提出。本文对单分子荧光共振能量转移技术在蛋白质与核酸分子构象动态变化、蛋白质-蛋白质相互作用以及蛋白质-核酸相互作用等方面取得的研究进展进行了综述。

Biomacromolecules participate in various kinds of vital processes. Observing and analyzing their structural dynamic and the dynamic processes of intermolecular interaction at molecular level is important for understanding the action mechanism. Since its advent,single molecular fluorescence resonance energy transfer（ SM-FRET） has demonstrated its great potential in studying the conformational change and interaction process of biomacromolecules,and a series of new mechanisms have been revealed. This review summarized recent progresses of SM-FRET in studying protein structural dynamic,nucleic acid structural dynamic,proteinprotein and protein-nucleic acid interactions.