外源GA和ABA对花生籽仁皱缩变异品系荚果生长的影响

Effect of Exogenous GA and ABA on Pod Growth of Seed Shrivel Mutant in Peanut

以荚果和籽仁生长发育正常的花生品系‘05D610’及其籽仁皱缩变异系‘05D677’为材料,在荚果膨大阶段初期滴加赤霉素(GA,20mg·L^(-1))和脱落酸(ABA,15 mg·L^(-1))处理幼果,检测荚果生长期间幼果或籽仁内源GA、ABA、ZT(玉米素)和IAA(生长素)含量的变化以及荚果和籽仁的生长量特征,以明确激素含量与荚果和籽仁生长发育的关系和‘05D677’籽仁皱缩的原因。结果显示:(1)外源施加GA处理,可显著提高‘05D610’和‘05D677’的内源GA和ZT含量,并推迟内源IAA含量峰值出现的时间,显著提高‘05D610’荚果和籽仁的鲜重、干重,极显著提高了‘05D677’荚果干重。(2)外源施加ABA处理,使‘05D610’内源GA含量显著降低,‘05D677’内源GA和ABA含量显著提高,同时两品系内源IAA含量峰值出现的时间推迟,使‘05D610’和‘05D677’籽仁干重分别显著和极显著提高。研究表明,用适宜浓度外源GA和ABA处理荚果膨大阶段的初期果针,可以调控荚果膨大阶段幼果或者籽仁中内源激素水平,从而提高花生荚果、籽仁产量;荚果膨大阶段内源GA、ABA和ZT含量不足是导致‘05D677’籽仁皱缩的重要原因。

In the early stage of pod enlargement,exogenous gibberellin（GA） and abscisic acid （ABA） were applied to young pods of peanut variety 05D610 with normal seed and its seed shrivel mutant 05D677.By measuring content change of endogenous GA,ABA,ZT （zeatin） and IAA （auxin）,as well as the change of growth characteristics in pods and seeds,we study the relationship between the pod or seed growth and hormone‘s contents,and analysis the causes of seeds hrinkage in 05D677.The main results were as fol-lows：（1 endogenous GA and ZT contents of pods and seeds were significantly increased,peak time of IAA content of that delayed,the dry weight of pod increased significantly,after exogenous GA treatment in 05D677.（2）Endogenous GA and ABA contents of pods and seeds were significantly increased,peak time of IAA content of that delayed,the dry weight of seeds increased significantly,after exogenous ABA treatment in 05D677.（3）In the stage of pods expanding,inadequate contents of GA,ABA and ZT in 05D677 pods or seeds lead to seeds shrinkage.