视黄醇结合蛋白的检测方法及临床应用

The Detection Method and Clinical Application of Retinol Binding Protein

视黄醇结合蛋白是人体血液中的转运蛋白,以视黄醇和蛋白结合的形式存在,一旦复合物中的视黄醇和靶细胞结合,极有可能导致视黄醇结合蛋白和蛋白分离,由肾小球滤出或肾小管细胞吸收。近年研究显示,视黄醇结合蛋白水平能真实、敏感的反映肝功能、肾小管功能损伤度,是反映营养状况、肾脏状况和疾病发展及转归的重要指标。当前,临床检测视黄醇结合蛋白的方法比较多,如放射免疫分析、免疫比浊、免疫电泳、酶联免疫吸附法等,其中,免疫比浊法对肝病、肾病早期诊断具有重大意义,具有操作简便、灵敏度高、实用性强的优势。下面,本文从视黄醇结合蛋白的检测方法出发,总结视黄醇结合蛋白的临床应用。

Retinol-binding protein is a transport protein in human blood, it exists in the form of retinol and protein binding. Once retinol in the complex binds to target cells, it is very likely to cause the separation of retinol-binding protein and protein. By glomerular filtration or tubular cell absorption. Recent studies have shown that retinol-binding protein levels can truly and sensitively reflect liver function and renal tubular injury, and are important indicators of nutritional status, kidney status, and disease development and prognosis. At present, there are many methods for clinical detection of retinol-binding proteins, such as radioimmunoassay, immunoturbidimetry, immunoelectrophoresis, and enzyme-linked immunosorbent assay. Among them, immunoturbidimetric assay is of great significance in the early diagnosis of liver diseases and nephropathy. Simple operation, high sensitivity and strong practicality. In the following, this article starts with the detection method of retinol binding protein and summarizes the clinical application of retinol binding protein.