真武汤联合紫杉醇新辅助化疗对宫颈癌病灶恶性程度的影响

Effect of Zhenwu decoction combined with paclitaxel neoadjuvant chemotherapy on the malignant degree of cervical cancer

目的:研究真武汤联合紫杉醇新辅助化疗对宫颈癌病灶恶性程度的影响。方法:选择在南充市中心医院及遂宁市中心医院接受新辅助化疗的宫颈癌患者,随机分为两组,观察组接受新辅助化疗联合真武汤、对照组接受新辅助化疗。化疗前及化疗2个疗程后,取血清并测定肿瘤标志物的含量;化疗后,取手术切除的宫颈癌病灶并测定增殖基因、抑癌基因、侵袭基因的mRNA表达量。结果:化疗2个疗程后,两组血清中CA12-5、bFGF、SCC-Ag的含量均低于化疗前且观察组血清中CA12-5、bFGF、SCC-Ag的含量以及宫颈癌病灶内Ki-67、TK-1、SOX-2、CyclinD1、S100A6、N-cadherin、Gal-3、CatL、CatD的mRNA表达量均低于对照组,宫颈癌病灶内p53、p16、FHIT、NF2-1、LATS1的mRNA表达量均高于对照组。结论:真武汤联合紫杉醇新辅助化疗能够较单纯新辅助化疗更为有效的杀伤宫颈癌细胞、降低宫颈癌病灶的恶性程度。

Objective： To study the effect of Zhenwu decoction combined with paclitaxel neoadjuvant chemotherapy on the malignant degree of cervical cancer. Methods： Patients with cervical cancer who accepted neoadjuvant chemotherapy in Nanchong Central Hospital and Suining Central Hospital between March 2015 and June 2017 were selected and randomly divided into two groups, the observation group received neoadjuvant chemotherapy combined with Zhenwu decoction therapy, and the control group received neoadjuvant chemotherapy. Before chemotherapy and after 2 courses of chemotherapy, the serum was collected to detect the contents of tumor markers; after chemotherapy, the surgically removed cervical cancer lesion was collected to detect the mRNA expression of proliferation genes, tumor suppressor genes and invasion genes. Results： After 2 course of chemotherapy, CA12-5, bFGF and SCC-Ag levels in serum of both groups were lower than those before treatment, and CA12-5, bFGF and SCC-Ag levels in serum as well as Ki-67, TK-1, SOX-2, CyclinD1, S100A6, N-cadherin, Gal-3, CatL and CatD mRNA expression in cervical cancer lesion of observation group were lower than those of control group whereas p53, p16, FHIT, NF2-1 and LATS1 mRNA expression in cervical cancer lesions were higher than those of control group （ P 〈 0.05）. Conclusion： Zhenwu decoction combined with paclitaxel neoadjuvant chemotherapy can be more effective than neoadjuvant chemotherapy alone to kill cervical cancer cells and reduce the malignancy of cervical cancer.