膀胱癌的超声造影时间-强度曲线参数与癌细胞侵袭性生长、病灶内血管新生的相关性

The correlation between contrast-enhanced ultrasonography time-intensity curve parameters of bladder cancer with the invasive growth of cancer cells and the angiogenesis in the lesion

目的:研究膀胱癌的超声造影时间-强度曲线参数与癌细胞侵袭性生长、病灶内血管新生的相关性。方法:选择2015年3月~2017年4月期间在鄂州市中心医院接受手术切除治疗的膀胱癌患者作为研究对象,术前进行超声造影检查,绘制时间-强度曲线后计算膀胱癌组织和癌旁组织的参数造影剂到达时间(AT)、峰值时间(PT)、峰值强度(PI)、廓清时间(WT);术后取膀胱癌组织和癌旁组织并测定增殖、侵袭、血管新生分子的表达量。结果:膀胱癌组织的AT、PT、WT水平显著低于癌旁组织,PI水平以及Rce1、YAP、β-catenin、c-myc、CUGBP1、Annexin-II、uPA、CXCL10、CXCR3、ILK、VEGF、COX2、HIF-1α、NAC1的mRNA表达量显著高于癌旁组织;膀胱癌组织中Rce1、YAP、β-catenin、cmyc、CUGBP1、Annexin-II、uPA、CXCL10、CXCR3、ILK、VEGF、COX2、HIF-1α、NAC1的mRNA表达量与AT、PT、WT水平呈负相关,与PI水平呈正相关。结论:膀胱癌超声造影时间-强度曲线参数的变化对癌细胞侵袭性生长、病灶内血管新生具有评估价值。

Objective： To study the correlation between contrast-enhanced ultrasonography time-intensity curve parameters of bladder cancer with the invasive growth of cancer cells and the angiogenesis in the lesion. Methods： Patients with bladder cancer who underwent surgical resection in Ezhou Central Hospital between March 2015 and April 2017 were selected as the research subjects. They received preoperative contrast-enhanced ultrasonography, the time-intensity curve was drawn, and the parameters AT, PT, WT and PI of bladder cancer tissue and adjacent tissue were calculated; after surgery, the bladder cancer tissue and adjacent tissue were collected to measure the expression of proliferation, invasion and angiogenesis molecules. Results： AT, PT and WT levels in bladder cancer tissue were significantly lower than those in adjacent tissue whereas PI level as well as Rce1, YAP, β-catenin, c-myc, CUGBP1, Annexin-II, uPA, CXCL10, CXCR3, ILK, VEGF, COX2, HIF-1α and NAC1 mRNA expression was significantly higher than those in adjacent tissue; Rce1, YAP, β-catenin, c-myc, CUGBP1, Annexin-II, uPA, CXCL10, CXCR3, ILK, VEGF, COX2, HIF-1α and NAC1 mRNA expressions in bladder cancer tissue were negatively correlated with the AT, PT and WT levels, and were positively correlated with the PI level. Conclusion： The change of the contrast-enhanced ultrasonography time-intensity curve parameters of bladder cancer is valuable for evaluating the invasive growth of cancer cells and the angiogenesis in the lesion.