柱层析提取法提取青蒿素的工艺研究

Column Chromatographic Extraction of Artemisinin from Artemisia annua

研究开发了一种柱层析提取法提取青蒿素的新工艺.将干燥粉碎的黄花蒿植物材料用提取溶剂V石油醚∶V95%乙醇=2∶8按m料∶V液=1.0∶3.5湿法装入层析柱中,静置1 h后洗脱.少量和放大提取实验都显示:收集3.50、4.75、7.00和10.50倍体积洗脱液时,青蒿素的提取率分别超过90%、95%、97%和99%.结果表明:该提取方法工艺简单、提取率高、溶剂用量少、节能环保、设备和生产成本低,适合工业上提取制备青蒿素.

A new column chromatographic extraction（ CCE） method was developed for the extraction of artemisinin from Artemisia annua. Dried material was loaded into a column using 3.5-fold（ V/m） extraction solvent of petroleum ether： 95% ethanol（ 2∶ 8）. After 1 h when artemisinin is fully dissolved,the column was eluted with the extraction solvent. The extraction efficiency in both small-scale and enlarged-scale experiments reached more than 90%,95%,97% or 99% when collecting 3.50-,4.75-,7.00-or 10.50-fold eluent,respectively. The results indicated that the CCE method is simple and highly efficient; and the extraction process can be completed in a column at room temperature at low equipment and production costs. It can be used for industrial extraction of artemisinin.