宫颈癌病人外周血调节性T细胞的表达水平及其临床意义

The expression level of regulatory T cells in peripheral blood of patients with cervical cancer,and its clinical significance

目的:探讨CD4+CD25+CD127-调节性T细胞(Treg)在宫颈癌病人外周血中的表达水平及其临床意义。方法:回顾性分析65例经病理检查确诊为宫颈癌病人的临床资料,归入宫颈癌组;同时选取同期60例子宫肌瘤病人及60名健康女性为对照,分别归入子宫肌瘤组和健康对照组。采用流式细胞仪检测研究对象外周血中CD4+CD25+CD127-Treg占CD4+CD25+细胞数量的百分比,比较3组间的差异;分析宫颈癌病人CD4+CD25+CD127-Treg外周血表达水平与宫颈癌临床分期、肌层浸润深度、病理分化程度及有无淋巴结转移等临床病理特征的相关性。结果:宫颈癌组的外周血CD4+CD25+CD127-Treg/CD4+CD25+百分比显著高于子宫肌瘤组和健康对照组(P<0.01),子宫肌瘤组亦明显高于健康对照组(P<0.01)。CD4+CD25+CD127-Treg/CD4+CD25+T百分比在宫颈癌临床分期、肌层浸润深度、病理分化程度及淋巴结有无转移间差异均有统计学意义(P<0.05~P<0.01)。结论:CD4+CD25+CD127-Treg表达水平与宫颈癌病变的发生、发展具有一定相关性,可初步评估宫颈癌的进展情况,具有重要临床意义。

Objective： To investigate the expression level of CD4^＋CD25^＋CD127^-regulatory T cells（ Treg） in peripheral blood of patients with cervical cancer,and its clinical significance. Methods： The clinical data of 65 patients with cervical cancer were retrospectively analyzed,and set as the cervical cancer group. Sixty patients with uterine myoma and 60 healthy women were divided into the uterine myoma group and control group,respectively. The proportions of CD4^＋CD25^＋CD127^-Treg to CD4^＋CD25^＋T cells of peripheral blood were detected using flow cytometry,and the difference of which between three groups was compared. The correlations of the expression level of CD4^＋CD25^＋CD127^-Treg in peripheral blood with the cancer clinical stage,myometrial invasion,tumor differentiation and lymph node metastasis were analyzed. Results： The ratio of CD4^＋CD25^＋CD127^-Treg to CD4^＋CD25^＋T cells in peripheral blood in cervical cancer group was significantly higher than that in other two groups（ P〈0. 01）,and which in uterine myoma group was significantly higher than that in control group（ P〈0. 01）. The differences of the ratio of CD4^＋CD25^＋CD127^-Treg/CD4^＋CD25^＋T cells in different clinical stage,pathological grade,infiltration depth and lymph node metastasis were statistically significant（ P〈0. 05 to P〈0. 01）. Conclusions： The expression level of CD4^＋CD25^＋CD127^-Treg is correlation with the incidence and development of cervical cancer in some degree,which has important significance in evaluating the progress of cervical cancer.