一种高效的蝴蝶兰花梗诱导丛生芽体系

An Efficient System for Inducing Cluster Buds of Phalaenopsis Pedicels

为提高蝴蝶兰组织培养效率,分别以幼嫩花梗和已开花蝴蝶兰花梗腋芽为外植体,采用两种消毒剂HgCl2、NaClO,3种消毒方式0.1%HgCl2,10min;10%NaClO,10min;5%NaClO,10min为处理,以VW(大量)+MS(微量、铁盐、有机)+100g·L-1马铃薯+0.1g·L-1 Ga3(PO4)2+6mg·L-16-BA+0.2mg·L-1 NAA为诱导基本培养基;VW(大量)+MS(铁盐、有机、微量)+100g·L-1马铃薯+3mg·L-16-BA+0.2mg·L-1 NAA为增殖分化培养基;1/2MS+0.1mg·L-1 NAA为生根培养基诱导丛生芽,拟建立以蝴蝶兰花梗腋芽为外植体的高效丛生芽诱导体系。结果表明:在0.1%HgCl2,10min消毒方式下,幼嫩花梗腋芽的污染率为0,丛生芽诱导率为95.6%;增殖率为216%;在生根培养基1/2MS+0.1mg·L-1 NAA上生根率为95.6%,建立了高效的蝴蝶兰幼嫩花梗腋芽诱导丛生芽。

In order to improve the efficiency of tissue culture of Phalaenopsis,we used the axillary buds of young and flowering butterfly orchids as explants,and adopted two disinfectants（HgCl2 and NaClO）,and three disinfection methods（0.1%HgCl2,10 min;10%NaClO,10 min;5% NaClO,10 min）to compare the disinfection effects.The mediums were as follows,VW（A large number of elements）＋ MS（trace,iron,organic）＋100 g·L^-1 potato＋0.1 g·L^-1 Ga3（PO4）2＋6 mg·L^-16-BA＋0.2 mg·L^-1 NAA as the basic medium,VW（A large number of elements）＋MS（trace,iron,and organic）＋ 100 g·L^-1 potato＋ 3 mg·L^-1 6-BA＋ 0.2 mg·L^-1 NAA as proliferation and differentiation medium,and 1/2 MS ＋0.1 mg·L^-1 NAA induced clustered buds for rooting medium.The results showed that the induction rates of pollution rate was 0,the young shoots of axillary buds were 95.6%,the reproducibility rate was 216%,and the rooting rate of 1/2 MS＋0.1 mg·L^-1 NAA in the rooting medium was 95.6%,under 0.1% HgCl210 min disinfection method indicated the use of two kinds of medium,disinfection methods and induced proliferation and differentiation,was suitable for inducing shoots of axillary buds of young peduncle.