三明治结构rGO/Fe_3O_4@mSiO_2载体的构筑及协同抗肿瘤性能

Construction and Synergistic Anticancer Efficacy of Sandwich-like rGO/Fe_3O_4@mSiO_2 Drug Carriers

采用温和的反应条件,制备出三明治结构rGO/Fe_3O_4@mSiO_2,利用SEM、TEM、FTIR、XRD和N_2吸附-脱附等对其形貌和性能进行表征,考查了其对Hela细胞的毒性和细胞荧光成像效果,并探讨了其形成机理。实验结果表明:rGO/Fe_3O_4@mSiO_2具有较高的比表面积(217 m^2·g^(-1)),对抗癌药物五氟尿嘧啶(5-FU)的载药率达到57.34%;它还具有较好的磁性,磁饱和强度为32 emu·g-1;而且rGO/Fe_3O_4@mSiO_2纳米复合物在光照条件下具有优异的光热转换性能,对He La细胞表现出明显的杀伤效果。

A novel sandwich-like nanocomposites of rGO/Fe3O4@mSiO2 were successfully fabricated through a facile method. The morphology and properties of nanocomposites were characterized by SEM, TEM, FTIR, XRD and N2 adsorption-desorption. As a result, it was observed that the nanocomposites exhibit the saturation magnetization of 32 emu·g^（-1）, and possess abundant pores with a large surface area as much as 217 m2·g-1. The loading capacity of Pentafluorouracil（5-FU） with the nanocomposites was high（57.34%）. The formation mechanism of rGO/Fe3O4@mSiO2 was discussed. Additionally, the synergistic anticancer efficacy of as-prepared nanocomposites against He La cell line was investigated by MTT assay and cell fluorescence microscopic imaging analysis. The results indicated that 5-FU loaded in the nanocoposites is effectively delivered to tumor lesion sites under the action of magnetic targeting. Moreover, the rGO/Fe3O4@mSiO2 nanocomposites have excellent photothermal conversion properties under light conditions, and show significant killing effect on He La cells.