不同品级红火蚁体内生物碱含量的比较

Comparison of venom alkaloids in different castes of individual ants of Solenopsis invicta Buren

本文旨在比较不同有机溶剂,不同浸泡时间对红火蚁体内生物碱的提取效率以及同巢蚁群中不同品级的红火蚁体内生物碱之间的差异。选用红火蚁整体浸泡法,获取生物碱样品,利用GC-FID技术进行定量分析。结果表明利用正己烷浸泡1 h即可获得红火蚁体内大部分的生物碱。同巢蚂蚁种群中,雌性生殖蚁体内生物碱含量最高,约为82μg/头,而工蚁体内的生物碱含量与个体大小呈正相关。

This study aimed to evaluate the effect of different organic solvents and extraction time on extraction efficiency of venom alkaloids from the fire ant,Solenopsis invicta. Meanwhile,the difference in alkaloids quantity from different castes of S. invicta was compared. The whole body soaking method with organic solvents was used to obtain venom alkaloids,and GC-FID technique was used for quantitative analysis of alkaloids. The results showed that the alkaloids in fire ant workers could be obtained by soaking the whole body in n-hexane for 1 h. Further,the content of venom alkaloids in alate ants was the highest,which was about 82 μg per ant. In worker ants,the alkaloids content was positively correlated with the worker size.