三阴性乳腺癌TCL诱导THP-1细胞活化及分化的研究

THP-1 activation and differentiation induced by TCL in triple negative breast cancers

目的:研究三阴性乳腺癌细胞裂解物(TCL)对单核细胞活化和分化所产生的影响,以及裂解物诱导单核细胞分化的机制。方法:选取三阴性乳腺癌细胞系HCC1937制备细胞裂解物,以人单核细胞系THP-1作为研究对象。将HCC1937细胞裂解物作用于THP-1细胞,检测细胞培养上清液中TNF-α、IL-12的分泌情况,并确定促进细胞因子分泌的最佳裂解物剂量和时间点。流式检测HCC1937细胞裂解物作用的THP-1细胞形态及细胞表面CD68表达的改变,并利用Q-PCR的方法检测裂解物作用后THP-1细胞中促分化关键因子转录因子CCAAT/增强子结合蛋白α(C/EBPα)和PU.1蛋白的表达情况。结果:HCC1937细胞裂解物活化的THP-1细胞,大量合成分泌TNF-α、IL-12(P<0.05)。THP-1细胞在裂解物作用后细胞体积、颗粒度等会发生增大,细胞表面CD68表达上调(P<0.05),上述表现符合巨噬细胞的特征。与此同时,作用后THP-1细胞中C/EBPα表达出现上调(P<0.05),而PU.1表达出现下调(P<0.05)。结论:三阴性乳腺癌HCC1937细胞裂解物活化人单核细胞THP-1,并通过促进分化因子C/EBPα的表达和抑制PU.

Objective： To investigate the activation and differentiation mechanisms of human THP-1 monocytes stimulated tumor cell lysate（TCL） in triple negative breast cancers. Methods：HCC1937 cells of triple negative breast cancers were used to produce TCL,and human monocyte THP-1 was used as a research object. The HCC1937 cell lysate was co-cultured with THP-1,and TNF-α and IL-12 levels in the cell culture supernatant were measured to determine optimum dose and time point of HCC1937 cell lysate affecting THP-1 activation. Then the cellular morphology change and CD68 expression in THP-1 cells were detected using flowcytometry. Quantitative PCR was performed to analyze C/EBPα and PU. 1 expression in THP-1 cells activated by HCC1937 cell lysate. Results：HCC1937 cell lysate induced THP-1 cells to secrete TNF-α and IL-12 in large quantity（ P 〈0. 05）. The cell volume and granularity of THP-1 stimulated by HCC1937 cell lysate were obviously increased（ P〈 0. 05）,and CD68 as well as C/EBPα expression on THP-1 cells was spontaneously up-regulated（ P 〈0. 05）,whereas PU. 1 was decreased（ P 〈0. 05）. Conclusion：HCC1937 cell lysate of triple negative breast cancers can activate human monocyte THP-1 and induce it＇s differentiation through boosting C/EBPα expression,yet inhibiting PU. 1.