北京地区南瓜病毒病病原种类鉴定

Identification of Pathogen Species of Virus Diseases Infecting Squash in Beijing Area

为明确北京地区南瓜病毒病种类及其主要侵染病原,2016~2017年在北京周边采集疑似感染病毒的南瓜病样84份,并根据南瓜上的6种病毒特异性引物对其进行反转录PCR(RT-PCR)检测。结果表明:共有79份南瓜病样检测显示阳性,其中黄瓜花叶病毒(Cucumber mosaic virus,CMV)的检出率最高,为52.38%;其次是小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)和西瓜花叶病毒(Watermelon mosaic virus,WMV),检出率分别为44.01%、14.29%,其他病毒暂未检出。此外,16.67%的样品受2种病毒复合侵染,CMV和ZYMV复合侵染占7.14%,ZYMV和WMV复合侵染占9.52%。北京地区南瓜上优势病毒种类为CMV,且存在病毒复合侵染现象。

In order to clear and definite species of squash virus diseases in Beijing area and their main infection pathogens,this paper collected 84 squash samples with suspected virus-like symptoms during 2016-2017 from Beijing area,and carried out detection by RT-PCR used 6 pairs of specific virus primers.The results showed that 79 samples were positive,among which Cucumber mosaic virus（CMV） had the highest detection rate-52.38%,followed by Zucchini yellow mosaic virus（ZYMV） and Watermelon mosaic virus（WMV）.Their detection rate were 44.01% and 14.29%,respectively.Other viruses were not yet detected.In addition,16.67% samples were mix-infected by 2 kinds of viruses.Mix infection of CMV and ZYMV took 7.14%.Mix infection of ZYMV and WMV took 9.52%.CMV was the prevalent pathogenic virus in Beijing area,where the mix infection of viruses on squash was existed.