24%苯唑草酮·烟嘧磺隆·莠去津可分散油悬浮剂高效液相色谱分析

Analysis of Topramezone+Nicosulfuron+Atrazine 24% OD by HPLC

建立高效液相色谱法,采用乙腈和水(0.05%磷酸水溶液)为流动相,使用反相色谱柱C8和紫外可变波长检测器,在245 nm波长下对24%苯唑草酮·烟嘧磺隆·莠去津可分散油悬浮剂进行分离和定量分析。结果表明:苯唑草酮、烟嘧磺隆、莠去津的线性相关系数分别为0.999 6、0.999 2和0.999 5,标准偏差分别为0.055、0.063和0.210,变异系数分别为2.59%、2.99%和1.03%,平均回收率分别为98.41%、99.54%和99.23%。该方法操作简单、快速、准确。

A method for separation and quantitative analysis of topramezone ＋ nicosulfuron ＋ atrazine 24% OD was established by HPLC, using acetonitrile and 0.05% phosphoric acid solution as mobile phase, on ZORBAX Eclipse Plus C_8 column and UV-absorbance detector, at 245 nm wavelength. The results showed that the linear correlation coefficients of topramezone, nicosulfuron and atrazine were 0.999 6, 0.999 2 and 0.999 5, the standard deviations were 0.055, 0.063 and 0.210, the variation coefficients were 2.59%, 2.99% and 1.03%, the average recoveries were 98.41%, 99.54% and 99.23%,respectively. The method was simple, rapid, accurate, and suitable for the qualitative and quantitative analysis.