分析保护剂在樱桃浓缩汁中农药残留检测的应用

Application of Analyte Protectants in Determination of Pesticide Residues in Cherry Condensed Juice

通过改进的QuEChERS样品前处理方法和气相色谱-串联质谱分析技术,研究樱桃浓缩汁中不同分析保护剂对24种农药的基质补偿作用。研究结果表明:山梨糖醇和古洛糖酸-γ-内酯在质量浓度大于2 g/L时,对大多数农药的基质效应为80%~130%。两者复配使用,可以减少分析保护剂的使用量,且对农药基质补偿效果明显。添加分析保护剂后,24种农药质量浓度为5~400μg/L时,线性关系良好,相关系数为0.987 4~0.999 7。24种农药添加回收率为67.4%~135.7%,RSD为0.9%~11.5%,方法定量限不超过4.746μg/L。

The matrix compensation of different analyte protectants for 24 pesticide residues in cherry condensed juice were analyzed by revised QuEChERS method and gas chromatography tandem mass spectrometric（GC-MS/MS）technology. The results showed that gulonic acid-γ-lactone and D-sorbitol could control the matrix effects of the majority pesticides in 80%-130%, when their concentration was more than 2 g/L. Their combination had obvious compensation effect. After applying the analyte protectants in determining of the 24 pesticide residues, good linearities were obtained in the range of 5-400 μg/L, with the coefficients of 0.987 4-0.999 7. The fortified recoveries of the 24 pesticides were 67.4%-135.7%, the relative standard deviations（RSD） were 0.9%-11.5%, and the limits of quantitation were less than 4.746 μg/L.