Foxp3在AOM/DSS诱导小鼠结肠癌模型中的表达及其意义

Expression of Foxp3 protein in AOM/DSS-induced colorectal cancer in mice and its significance

目的观察头状/翅状螺旋蛋白3(FOXP3)在氧化偶氮甲烷(azoxymethane,AOM)/葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导小鼠结肠癌中的表达水平及其意义。方法将雌性SPF级C57BL/6小鼠随机分为两组:对照组10只;AOM/DSS组(模型组)10只。模型组小鼠腹腔注射AOM(10 mg/kg),饮用1%DSS 1周,之后正常饮水2周,以上为1个周期,连续3个周期;对照组以等体积生理盐水腹腔注射,并正常饮水。于实验11周末处死所有小鼠。结肠病理组织HE染色观察病理组织学改变。收集血浆,血细胞分析仪检测单核细胞计数;留取结肠癌组织,免疫组织化学染色方法检测Foxp3蛋白表达水平。结果结肠组织行HE染色后发现模型组小鼠均出现不同程度的结肠癌表现。模型组血浆单核细胞计数明显低于正常组[(5.14±0.25)×10~9/L vs(5.78±0.17)×10~9/L,P<0.01],免疫组化染色半定量分析结肠癌组织中Foxp3蛋白表达水平较正常对照组显著升高(3.55±0.88 vs 1.00±0.71,P<0.05)。结论在AOM/DSS诱导的小鼠结肠癌中,Foxp3蛋白表达增加促进了其发生发展。

Objective To explore the Foxp3 protein expression and its clinical significance in colorectal cancer induced by azoxymethane（ AOM） plus dextran sulfate sodium（ DSS） in C57BL/6 female mice. Methods The female C57BL/6 mice were randomly divided into 2 groups（ n = 10 in each group） ： control group and AOM/DSS group（ model group）. The mice were intraperitoneally injected of AOM（ 10 mg/kg） in model group,and drank 1% DSS for 1 week and then normal water for 2 weeks as one cycle for three consecutive cycles. The mice in control group were given the same volume of normal saline and drank the normal water. AOM/DSS and control mice were sacrificed at the end of experiment. The pathological changes of colon carcinoma were observed by HE dyeing. The fresh plasma was collected for blood test analysis. The Foxp3 protein expression was detected by the IHC. Results HE staining showed that the mice in model group exhibited the symptoms of colorectal cancer. Compared with control group,the plasma level of monocyte in model group decreased significantly[（ 5. 78 ± 0. 17） × 10^9/L vs（ 5. 14 ± 0. 25） × 10^9/L,P〈0. 01],and the colonic expression of Foxp3 protein in model group increased significantly（ 1. 00 ± 0. 71 vs 3. 55 ± 0. 88,P〈0. 05）. Conclusion In AOM/DSS-induced experimental colorectal cancer mice,the expression of Foxp3 may promote the development.