摘要
目的探讨膝骨关节炎(KOA)合并骨质疏松(OP)患者血清β-catenin、DKK-1和基质金属蛋白酶-9(MMP-9)的水平及其相关性。方法采用酶联免疫吸附法(ELISA)对60例KOA合并OP患者(OA+OP组),60例KOA患者(OA组),60例OP患者(OP组)及35例体检健康的志愿者(正常组)血清β-catenin、DKK-1和MMP-9水平进行检测,并比较四组受试者血清β-catenin、DKK-1和MMP-9表达水平;分析OA+OP组患者血清β-catenin、DKK-1和MMP-9变化及与红细胞沉降率(ESR)、C-反应蛋白(CRP)、骨密度(BMD)相关性。结果 OA+OP组血清β-catenin水平显著低于正常组和OA组,高于OP组(P<0.05)。OA+OP组血清DKK-1、MMP-9水平显著高于正常组和OA组(P<0.05);OA+OP组血清MMP-9水平高于OP组(P<0.05)。相关性分析结果显示OA+OP组患者血清β-catenin、DKK-1与ESR、CRP呈正相关,MMP-9与ESR、CRP无相关性。β-catenin与腰椎、股骨颈骨密度(BMD)呈正相关,DKK-1、MMP-9与腰椎、股骨颈骨密度(BMD)呈负相关(P<0.05)。结论 OA+OP组患者血清β-catenin、DKK-1、MMP-9表达水平的失调与OA合并OP的发生发展密切相关,与临床指标ESR、CRP及BMD存在一定的相关性。
Objective To investigate the levels of serum β-catenin,DKK-1 and MMP-9 in knee osteoarthritis( KOA) combined with osteoporosis( OP) and their correlation with clinical indexes. Methods ELISA was used to detect the levels of serum β-catenin,DKK-1 and MMP-9 in 60 KOA patients combined with OP( OA + OP group),60 KOA patients( OA group),60 OP patients( OP group) and 35 healthy volunteers( control group). The levels of serum β-catenin,DKK-1 and MMP-9 were compared among the four groups. The correlations of serum β-catenin,DKK-1 and MMP-9 with ESR,CRP and BMD in KOA combined with OP were analyzed.Results The level of serum β-catenin in OA + OP group was significantly lower than that in control group and OA group,but higher than that in OP group( P〈0. 05). The levels of serum DKK-1 and MMP-9 in OA + OP group were significantly higher than those in control group and OA group( P〈0. 05). The level of serum MMP-9 in OA + OP group was higher than that in OP group( P〈0. 05).The serum β-catenin and DKK-1 were positively correlated with ESR and CRP in OA + OP group,while MMP-9 was not correlated with ESR and CRP. β-catenin was positively correlated with lumbar spine and femoral neck BMD,while DKK-1 and MMP-9 were negatively correlated with lumbar spine and femoral neck BMD( P〈0. 05). Conclusion The imbalance of the expression of serum β-catenin,DKK-1 and MMP-9 in OA + OP group is closely related to the occurrence and development of KOA combined with OP,and the expression has a certain correlation with the clinical indicators ESR,CRP and BMD.
作者
丁婷婷
江超
李季青
谢长好
李志军
陈琳洁
DING Tingting1, JIANG Chao2, LI Jiqin2, XIE Changhao1, LI Zhijun1, CHEN Linjie1(1.Department of Rheumatology and Immunology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China;2. Rheumatoid Immunology Laboratory, First Affiliated Hospital of Bengbu Medical Colleg)
出处
《山西医科大学学报》
CAS
2018年第3期289-293,共5页
Journal of Shanxi Medical University
基金
安徽省自然科学基金资助项目(1608085MH215)