摘要
The effect of various metal ions on the DNA mediated energy transfer between simultaneously bound drugs was investigated using spectroscopic methods.It was found that addition of divalent metal ions(Mg^(2+),Ca^(2+),Mn^(2+),Co^(2+)and Ni^(2+))resulted in further decrease of the ethidium fluorescence intensity,while a small increase was observed in the TMPyP emission band,implying that the energy of excited ethidium was transferred to TMPyP.This DNA-mediated quenching efficiency between ethidium and TMPyP was significantly enhanced by the presence of all metal ions.Among the divalent metal ions,alkali earth metal ions and Mn^(2+)displayed higher quenching efficiencies than other transition metal ions.The distances required to permit the energy transfer between the two drugs in DNA were calculated as 68,66,62,48and 38in the presence of100μmol·L^(-1 )of Mg^(2+),Ca^(2+),Mn^(2+),Co^(2+)and Ni ^(2+)ion,respectively.The disturbed binding conformation of TMPyP in DNA by metal ions presumably accounts for the difference.
The effect of various metal ions on the DNA mediated energy transfer between simultaneously bound drugs was investigated using spectroscopic methods. It was found that addition of divalent metal ions (Mg2+, Ca2+, Mn2+, Co2+ and Ni2+) resulted in further decrease of the ethidium fluorescence intensity, while a small increase was observed in the TMPyP emission band, implying that the energy of excited ethidium was transferred to TMPyP. This DNA-mediated quenching efficiency between ethidium and TMPyP was significantly enhanced by the presence of all metal ions. Among the divalent metal ions, alkali earth metal ions and Mn2+ displayed higher quenching efficiencies than other transition metal ions. The distances required to permit the energy transfer between the two drugs in DNA were calculated as 68, 66, 62, 48 and 38 in the presence of 100 μmol·L-1 of Mg2+, Ca2+, Mn2+, Co2+ and Ni2+ ion, respectively. The disturbed binding conformation of TMPyP in DNA by metal ions presumably accounts for the difference.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2018年第6期1970-1974,共5页
Spectroscopy and Spectral Analysis
基金
the National Research Foundation of Korea(20011-0013534,2009-0076930)
the National Natural Science Foundation of China(21265022)
关键词
光学
文摘
英文
Energy transfer
Divalent cations
DNA
Ethidium
TMPyP