苗药赤胫散提取物的体外抑菌活性研究

Study on in vitro Bacteriostatic Activity of the Extracts from Miao Medicine Polygonum runcinatum

目的:研究苗药赤胫散提取物的体外抑菌活性。方法:采用管碟法,以氯霉素和氟康唑为阳性对照,测定赤胫散的水提物和95%乙醇提取物及其乙酸乙酯、正丁醇部位萃取物对金黄色葡萄球菌、大肠埃希菌、福氏志贺氏菌、伤寒杆菌、枯草芽孢杆菌、绿脓杆菌、乙型溶血性链球菌、白色念珠菌、新生隐球菌等9种菌株的抑菌作用,筛选出具有抑菌作用的部位及对药物敏感的试验菌株。分别采用微量肉汤稀释法和琼脂培养基平板法,测定赤胫散95%乙醇提取物及其不同萃取部位对敏感菌株的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果:赤胫散的水提物几乎无抑菌活性;95%乙醇提取物对各菌株表现出不同程度的抑制作用,对伤寒杆菌等6种细菌的抑菌作用优于乙型溶血性链球菌及2种真菌;95%乙醇提取物的乙酸乙酯及正丁醇萃取部位表现出良好的抑菌作用,且乙酸乙醇萃取部位强于正丁醇部位,但对真菌均未见抑制作用;95%乙醇提取物对上述6种细菌的MIC、MBC分别为6.25~12.5、12.5~25 mg/mL,其乙酸乙酯部位的MIC、MBC分别为3.13~6.25、6.25~12.5 mg/mL,其正丁醇部位的MIC、MBC分别为6.25~12.5、12.5~25 mg/mL。结论:苗药赤胫散的95%乙醇提取物及其乙酸乙酯、正丁醇部位萃取物对伤寒杆菌等6种常见细菌具有明显的体外抑菌作用。

OBJECTIVE：To study in vitro bacteriostatic activity of the extracts from Miao medicine Polygonum runcinatum.METHODS：Using chloramphenicol and fluconazole as positive control,the inhibitory effects of water extract,95% ethanol extract and its ethyl acetate and n-butanol fraction on 9 kinds of strains were determined by cup plate method, such as Staphylococcus aureus,Escherichia coli,Shigella flexneri,Salmonella typhi,Bacillus subtilis,Pseudomonas aeruginosa,type B Hemolytic streptococcus,Candida albicans and Cryptococcus neoformans. The parts with bacteriostatic activity and trial strains sensitive to drug were screened. Micro-broth dilution method and agar culture medium plate method were used to determine MIC and MBC of 95% ethanol extract and its fractions of P. runcinatum to sensitive strains. RESULTS：The water extract of P.runcinatum almost had no inhibitory effect. 95% ethanol extract showed different degrees of bacteriostatic activity to strains;bacteriostatic effects of it to 6 kinds of bacteria as S. typhi was better than that of type B H. streptococcus and 2 kinds of fungus.The ethyl acetate and n-butanol fraction of 95% ethanol extracts showed good bacteriostatic effect and the ethyl acetate fraction had stronger effect,while all the fractions of 95% ethanol extracts showed no inhibitory effect on fungus. MIC and MBC of 95%ethanol extract to above 6 kinds of bacteria were 6.25-12.5 and 12.5-25 mg/mL,respectively;MIC and MBC of ethyl acetate fraction were 3.13-6.25, 6.25-12.5 mg/mL; MIC and MBC of n-butanol fraction were 6.25-12.5, 12.5-25 mg/mL.CONCLUSIONS：95% ethanol extract,ethyl acetate and n-butanol fractions of Miao medicine P. runcinatum have obvious in vitro bacteriostatic activity to 6 common bacterias as S. typhi.