非离子型聚丙烯酰胺单用及与纳米微粉硅胶联用对甘草次酸脂化乳稳定性的影响

Effects of Non-ionic Polyacrylamide Alone or Combined with Nanoscale Silica Gel on the Stability of Glycyrrhetinic Acid Lipo-emulsion

目的:考察非离子型聚丙烯酰胺(型号:NPAM 1400)单用及与纳米微粉硅胶(nSiO_2)联用对甘草次酸脂化乳稳定性的影响。方法:采用紫外-可见分光光度计,分别测定脂化乳离心前后稀释液在500 nm波长处的吸光度,以未加入稳定辅料的制剂为空白计算稳定性系数(K_E)和稳定性系数之比(K_E/K_(E空白)),评价加入不同浓度(200~600 mg/L)非离子型聚丙烯酰胺对甘草次酸脂化乳稳定性的影响,初步确定单用最佳浓度后,再考察其与不同浓度的n SiO_2[亲水性(0.2%、0.3%、0.4%)和疏水性(0.2%、0.3%、0.5%)]联用对甘草次酸脂化乳稳定性的影响。结果:单用时,加入300 mg/L非离子型聚丙烯酰胺的甘草次酸脂化乳的KE最小,K_E/K_(E空白)为0.22;联用时,加入300 mg/L非离子型聚丙烯酰胺+0.2%疏水性n SiO_2的甘草次酸脂化乳的KE最小,K_E/K_(E空白)为0.27。结论:单用非离子型聚丙烯酰胺及与n SiO_2联用均可在一定程度上提升甘草次酸脂化乳的稳定性,其中单用300 mg/L非离子型聚丙烯酰胺效果最佳。

OBJECTIVE：To investigate the effects of non-ionic polyacrylamide（model：NPAM 1400）alone or combined with nanoscale silica gel（n SiO2）on the stability of Glycyrrhetinic acid lipo-emulsion. METHODS：UV-spectrophotometer was used to determine absorbance of diluent at 500 nm before and after lipo-emulsion centrifugation. Using the preparation without stable excipient as blank,stability coefficient（KE）and it ratio（KE/K（E blank））were calculated. Effects of different concentrations（200-600 mg/L）of non-ionic polyacrylamide on the stability of Glycyrrhetinic acid lipo-emulsion were evaluated. The optimal concentration of non-ionic polyacrylamide alone was determined primarily,and then the effects of it combined with different concentrations of hydrophilic n SiO2（0.2%,0.3%,0.4%） or hydrophobic（0.2%,0.3%,0.5%） on the stability of Glycyrrhetinic acid lipo-emulsion were investigated. RESULTS：When adding non-ionic polyacrylamide alone,KEof Glycyrrhetinic acid lipo-emulsion mixed with 300 mg/L non-ionic polyacrylamide was the lowest,KE/K（E blank）was 0.22;when combined,KEof Glycyrrhetinic acid lipo-emulsion mixed with 300 mg/L non-ionic polyacrylamide and 0.2% hydrophobic n SiO2 was the lowest,KE/K（E blank）was 0.27.CONCLUSIONS：Non-ionic polyacrylamide alone or combined with n SiO2 all can promote the stability of Glycyrrhetinic acid lipo-emulsion,among which 300 mg/L non-ionic polyacrylamide alone is the best.