炮制方法及提取溶剂对何首乌中主要成分含量的影响

Effects of Processing Methods and Extraction Solvents on the Contents of Major Components in Polygonum multiflorum

目的:研究不同炮制方法及不同提取溶剂对何首乌中主要成分含量的影响。方法:采用黑豆汁蒸法和清蒸法对生何首乌进行炮制,分别用水、50%乙醇、70%乙醇、90%乙醇对生何首乌、黑豆汁制何首乌、清蒸制何首乌和市售制何首乌4种饮片进行提取,采用高效液相色谱法同时测定各样品中4种主要成分没食子酸、二苯乙烯苷、大黄素、大黄素甲醚的含量。结果:3种何首乌炮制品的4种提取液中4种主要成分的含量均较生品中更高;没食子酸以水提时含量最高,二苯乙烯苷以90%乙醇提取时含量最低,大黄素和大黄素甲醚以50%和70%乙醇提取时含量较高。结论:不同炮制方法和提取溶剂能明显影响何首乌中主要成分的含量;各炮制品中各成分的含量变化未呈现一定的规律性。

OBJECTIVE：To study the effects of different processing methods and extraction solvents on the contents of major components in Polygonum multiflorum. METHODS： Decoction of black soybean and water were used to steam the raw P.multiflorum. Water,50% ethanol,70% ethanol and 90% ethanol were used to extract the raw,black soybean steamed,water steamed and commercial processed P. multiflorum respectively. HPLC method was used to detect the contents of gallic acid,2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside（THSG）,emodin and physcion. RESULTS：The contents of 4 major components in 4 kinds of extracts from 3 kinds of processed P. multiflorum were higher than raw sample;the content of gallic acid extracted with water was the highest;the content of THSG extracted with 90% ethanol was the lowest;the contents of emodin and physcione extracted with 50% and 70% ethanol were the higher. CONCLUSIONS：Different processing methods and different extraction solvents had effect on the contents of the main compounds of P. multiflorum. The contents of each components in the processing products didn＇t show certain regularity.