不同温度条件下不同采血管对神经元特异性烯醇化酶检测结果的影响

Effect of different temperature conditions and different blood collection tube on the detection results of neuronspecific enolase

目的:探讨在不同温度下使用不同采血试管对神经元特异性烯醇化酶(NSE)检测结果的影响。方法:选取健康体检者40例,空腹采取静脉血,每份血液标本同时分装于4个试管,其中包括2管肝素抗凝管(绿色)、2管促凝剂分离胶管(黄管)。将2管肝素抗凝管标本离心,分别置于4℃和25℃于1 h、3 h、6 h、24 h检测NSE水平;分离胶管NSE检测方法同肝素抗凝管。结果:(1)肝素管组标本在4℃时不同时间点NSE检测结果差异无统计学意义(P>0.05),在25℃时NSE结果随保存时间延长而增高。6 h、24 h对应的4℃和25℃的NSE结果比较,差异有统计学意义(P<0.05)。(2)分离胶管组标本在4℃和25℃条件下不同时间点检测NSE结果差异无统计学意义(P>0.05)。(3)在4℃下肝素抗凝组和分离胶组对应不同时间点NSE测定结果差异无统计学意义(P>0.05)。结论:肝素管采集标本在4℃条件下保存对NSE检测结果无影响;分离胶管采集标本在4℃和25℃条件下保存对检测NSE结果保持稳定。

Objective：To explore the effect of different temperature conditions and different blood collection tube on the detection results of neuronspecific enolase（NSE）.Methods：40 health examiners were chosen,and the fasting venous blood was collected.Each blood specimen was simultaneously divided into 4 test tubes,including 2 tubes of heparin anticoagulant（green）,and 2 tubes of coagulant separation rubber tubes（yellow）.The specimens of 2 tubes of heparin anticoagulant were centrifuged and placed at 4℃and 25℃ at 1 hour,3 hours,6 hours,24 hours to detect NSE levels.The NSE detection method for separating rubber tube was the same as heparin anticoagulant tube.Results：（1）There was no significant difference between the NSE test results of the heparin tube group at 4℃ at different time points（P〉0.05）,at 25℃,the results of NSE increased with the prolongation of the preservation time.The results were statistically significant for the corresponding NSE results at 6 hours and 24 hours at 4℃ and 25℃（P〈0.05）.（2）There was no significant difference between the NSE test results of the separation tube group at 4℃ and 25℃ at different time points（P〉0.05）.（3）At 4℃,there was no significant difference in the results of NSE determination between the heparin tube group and the separation tube group at different time points（P〉0.05）.Conclusion：The samples collected from the heparin tube had no effect on the results of NSE detection at 4℃.The samples collected from the isolated tube were kept stable for the results of NSE at 4℃and 25℃.