加味龟鹿二仙胶汤联合顺铂对小鼠Lewis肺癌组织、骨髓组织TGF-β_1/Smad信号通路的影响

The Effect of Modified Guilu Erxianjiao Decoction Combined with Cisplatin on TGF-β_1/Smad Signaling Pathway in Lewis Lung Cancer Tissues and Bone Marrow

目的:观察加味龟鹿二仙胶汤联合顺铂对小鼠Lewis肺癌组织、骨髓组织TGF-β_1/Smad信号通路的影响。方法:建立气阴两虚型C57BL/6J小鼠Lewis肺癌模型,随机分为正常对照组、模型组、加味龟鹿二仙胶汤组、顺铂组及顺铂+加味龟鹿二仙胶汤低、中、高剂量组,每组20只,干预21 d。观察小鼠瘤质量、抑瘤率及骨髓有核细胞计数,Western Blot检测各组移植瘤组织及骨髓组织中TGF-β_1、Smad4和Smad7的蛋白表达。结果:药物干预后,与模型组比较,联合组肿瘤质量均显著降低,移植瘤组织中TGF-β_1、Smad4表达显著降低,Smad7表达显著升高,骨髓组织中TGF-β_1、Smad4、Smad7表达均显著降低(P<0.01)。加味龟鹿二仙胶汤组骨髓有核细胞计数显著高于模型组(P<0.01)。结论:加味龟鹿二仙胶汤与顺铂联用可显著抑制肿瘤增长,提高抑瘤率,减轻骨髓抑制。其机制可能是通过调节TGF-β_1/Smad信号通路来实现。

Objective：To observe the effect of modified Guilu Erxianjiao decoction combined with cisplatin on TGF-β1/Smad signaling pathway in lewis lung cancer tissues and bone marrow. Methods：Lewis lung cancer model of C57BL/6J mice with deficiency of both vital energy and yin were established and randomly divided into control group, model group, modified Guilu Erxianjiao decoction group, cisplatin group ,cisplatin＋modified Guilu Erxianjiao decoction in the low-, medium-and high-dose groups （combined groups ） , with 20 mice in each group. After intervention for 21 d, the tumor mass, tumor inhibition rate and bone marrow nucleated cell count were ob- served. The protein expression of TGF-β1, Smad4 mad Smad7 in each group of transplanted tumor and bone marrow were detected by Western blot. Results：Compared with the model group, the tumor mass decreased significantly（ P〈0. 01 ）. In the lung cancer tissues ,the protein expression of TGF-β1 and Smad4 were significantly decreased, and the protein expression of Smad7 was significantly increased. In the bone marrow tissue, the protein expression of TGF-β1, Smad4 and Smad7 was significantly decreased（P〈0.01 ）. Conclusion：Modified Guilu Erxianjiao decoction combined with cisplatin can significantly inhibit tumor growth,improve tumor inhibition rate, and reduce bone marrow suppression. The mechanism may be achieved by modulating the TGF-β1/Smad signaling pathway.