犬急性结肠炎模型建立方法初探

Primary investigation on establishment method of canine acute colitis model

为了探讨犬急性结肠炎模型的建立方法,采用10%乙酸按体重10 m L/kg对试验犬进行深部灌肠以建立犬急性结肠炎模型,并通过临床症状观察及彩色多普勒超声诊断仪、结肠内窥镜和组织病理学方法进行检查确诊。结果表明：试验犬建模后均出现精神变差、食欲废绝,排水样粪便并且次数较多的症状;B型超声波检查可见结肠壁增厚、分层不清,呈弥散性的低回声,肠腔变窄;结肠内窥镜检查可见降结肠黏膜充血、水肿明显,表面有白色的伪膜,并有片状糜烂,伴有出血;组织病理学检查可见结肠黏膜结构不完整,黏膜层仅残存有少量的上皮细胞,肠隐窝减少甚至消失,杯状细胞明显减少,黏膜下层水肿、增厚,出现大量增生的小血管。说明10%乙酸按体重10 m L/kg采用深部灌肠的方法可以建立犬急性结肠炎模型。

In order to explore the method to establish canine acute colitis model, experimental dogs were given 10% acetic acid at a dose of 10 mL/kg via deep enema for the establishment of canine acute colitis model. The model was examined and was confirmed via elinical symptom observation, color doppler ultrasound equipment （B-mode ultrasound）, colonoseopy and histopathology. The results showed that clinical symptoms containing depression,loss of appetite, frequent diarrhea appeared on experimental clogs after modeling. B-mode ultrasound features were colon wall thickening and unclear layering, diffuse weak echo, and lumen narrowing. Colonoseopy displayed obvious mucosa hyperemia and edema, white pscudomembrane covered,with sheet erosion and hemorrhage. Histopathological examination showed incomplete mucosal structure, only a few epithelium remaining in the mucosa, reduced or even eliminated crypts, significantly reduced goblet cells, ssbmucosa edema and thickening with a large number of proliferating small vessels. The results suggested that 10% acetic acid at a dose of 10 mL/kg via deep enema can lee used to establish canine acute colitis model.