GINS2基因过表达对K562细胞增殖的影响

Effect of over-expression of GINS2 gene on proliferation of K562 cells

目的检测GINS2基因过表达对K562细胞增殖的影响。方法将Ad-GINS2感染K562细胞,扩增腺病毒,同时设空载体转染的空载组和未感染病毒的未处理组,PCR及Western blot法检测细胞中GINS2的转录及表达水平,MTT法检测细胞增殖水平。结果与空载组和未处理组相比,Ad-GINS2感染组细胞GINS2基因转录水平及蛋白表达水平显著升高(P<0.05),Ad-GINS2能明显促进细胞增殖(P<0.05)。结论 GINS2基因的重组腺病毒载体成功感染K562细胞并可促进K562细胞增殖,为进一步明确GINS2基因在白血病中的作用奠定了基础。

Objective To investigate the effect of over-expression of GINS2 gene on proliferation of K562 cells. Methods K562 cells were infected with Ad-GINS2 for amplification of adenovirus,using those transfected with empty vector and those uninfected as control. The m RNA transcription and protein expression levels of GINS2 were determined by PCR and Western blot respectively,while the proliferation level of K562 cells by MTT assay. Results Both the m RNA transcription and protein expression levels of GINS2 in K562 cells infected with Ad-GINS2 increased significantly（P〈0. 05）,and Ad-GINS2 promoted the cell proliferation significantly（P〈0. 05）. Conclusion Recombinant adenovirus with GINS2 gene infected K562 cells and promoted the cell proliferation successfully,which laid a foundation of understanding the role of GINS2 gene leukemia.