吖啶橙染色法用于湖北钉螺血淋巴细胞的观察

Observation of hemocytes of Oncomelania hupensis by acridine orange staining

将湖北钉螺软体组织挤压后获取血淋巴液制成涂片,采用荧光染料吖啶橙染色,荧光显微镜下观察血淋巴细胞。结果可见12种形态的血淋巴细胞:红色多浆型无颗粒圆形淋巴细胞、红色多浆型有颗粒圆形淋巴细胞、红色少浆型无颗粒圆形淋巴细胞、红色少浆型有颗粒圆形淋巴细胞、绿色多浆型无颗粒圆形淋巴细胞、绿色多浆型有颗粒圆形淋巴细胞、绿色少浆型无颗粒圆形淋巴细胞、绿色少浆型有颗粒圆形淋巴细胞、多浆伸展型淋巴细胞、少浆伸展型淋巴细胞、红色胞质梭形淋巴细胞、绿色胞质梭形淋巴细胞,表明该方法可用于湖北钉螺血淋巴细胞的染色,为探讨贝类免疫防御、研究灭螺药物或各种生物与理化因素对湖北钉螺血淋巴细胞的影响提供技术支持。

Hemolymph smears were prepared after extruding soft tissues of Oncomelania hupensis and stained by fluorescent dye acridine orange. The hemocytes were observed under a fluorescence microscope. The following 12 types of hemocytes were clearly seen, including round hemocytes with abundance of red cytoplasm, round hemocytes with abundance of red cytoplasm and intracellular granules, round hemocytes with little red cytoplasm, round hemo-cytes with little red cytoplasm but the presence of intracellular granules, round hemocytes with abundance of green cytoplasm, round hemocytes with abundance of green cytoplasm and intracellular granules, round hemocytes with little green cytoplasm, round hemocytes with little green cytoplasm but the presence of intracellular granules, hemocytes with abundance of cytoplasm and pseudopodia, hemocytes with little cytoplasm but the presence of pseudopodia,fusiform hemocytes with red cytoplasm, and fusiform hemocytes with green cytoplasm. The results indicated that the method could be used for the staining of hemocytes of O. hupensis, which will provide technical support for studies on shellfish immune defense and the effects of various biological, physical and chemical factors on hemocytes of O.hupensis.