摘要
目的:脂多糖是否对牙周膜间充质干细胞(periodontal ligment stem cells,PDLSCs)中组蛋白去甲基化酶相关基因表达存在调控功能。方法:利用不同剂量的脂多糖对牙周膜干细胞进行刺激处理;在mRNA水平利用实时定量RT-PCR(real time reverse transcription-polymerase chain reaction,qRT-PCR)检测组蛋白去甲基化酶相关基因的表达。qRT-PCR结果显示HR(hairless homolog)基因在脂多糖刺激后24h和48h结果显示,能促进HR的表达,其表达升高的水平与脂多糖刺激时间有相关性。1、5和10mg/L脂多糖在作用48h后都能促进HR的表达,其表达升高的水平与脂多糖刺激剂量有相关性。10μg/mL脂多糖抑制牙周膜干细胞体外矿化能力。结论:在牙周膜干细胞中脂多糖对牙周膜干细胞组蛋白去甲基化酶HR的表达有促进作用。
Objective:To investigate whether LPS could regulate the expression of histone demethylases in periodontal ligament stem cells(PDLSCs).Methods:Different doses of LPS were used to stimulate the PDLSCs.The qRT-PCR was used to detect the expression of histone demethylases at the mRNA level.Results:The qRT-PCR results showed that the expression levels of HR were up-regulated at 24 and 48 hafter LPS treatment in a time dependent manner.And 1,5,and 10μg/mL LPS could increase the expression level of HR in a dose dependent manner at 48 h.10μg/mL LPS inhibited the mineralization of PDLSCs.Conclusion:LPS could induce the expression level of histone demethylase HR in PDLSCs.
作者
张琛
姚睿
范志朋
ZHANG Chen;YAO Rui;FAN Zhi-peng(Tianjin Medical University School, Tianjin, 300070, China;Department of Pediatric Dentistry, Stomatological Hospital of Tianjin, 300041, China;Beijing Key Laboratory of Tooth Regenera tion and Function Reconstruction ,School of Stomatology ,Capital Medical University ,Beijing, 100050 ,China.)
出处
《口腔医学研究》
CAS
北大核心
2018年第6期648-652,共5页
Journal of Oral Science Research
基金
北京市自然科学基金(编号:7172089)