箭根酮对LPS刺激牙周膜成纤维细胞增殖能力的影响

Effects of Taccaloniolde on LPS-stimulated Proliferation of Periodontal Ligament Fibroblasts

目的:研究箭根酮对LPS刺激牙周膜成纤维细胞增殖能力的影响及机制研究。方法:将人牙周膜成纤维细胞培养在含LPS的培养液中,同时加入或不加不同浓度的箭根酮,同时设不含箭根酮的空白对照组,24、48、72h后,以CCK-8法检测细胞的增殖情况。流式检测细胞凋亡情况。结果:加药培养24、48、72h后,不同浓度箭根酮组细胞的相对活性明显低于对照组和LPS刺激组,结果有统计学意义(P<0.001)。流式结果表明,相比于对照组,箭根酮能够显著促进细胞凋亡。结论:箭根酮对LPS刺激的牙周膜成纤维细胞增殖具有明显抑制作用,且其可能机制为促进细胞凋亡。

Objective：To research the effect and mechanism of taccaloniolde on LPS-stimulated proliferation of human periodontal ligament fibroblasts.Methods：Human periodontal ligament fibroblasts were cultured in LPScontaining medium with or without different concentrations of taccaloniolde.At the same time,the blank control group without taccaloniolde was established.After 24,48,and 72 hours,the cell proliferation was detected by CCK-8 assay and the cell apoptosis was detected by flow cytometry.Results：After 24,48,and 72 hof drug addition,the relative cell viability in the different doses of taccaloniolde was significantly lower than that stimulated with the control and LPS.Compared with the control group,taccaloniolde could promote apoptosis significantly.Conclusion：Taccaloniolde can significantly inhibited cell proliferation of human periodontal ligament fibroblasts increased by LPS-stimulated,and its possible mechanism is due to promote apoptosis.