谷子LEA＿1基因家族鉴定及其渗透胁迫响应

Identification and the Response to Osmotic Stress of LEA＿1 Gene Family in Setaria italica

胚胎发育晚期丰富(late embryogenesis abundant,LEA)蛋白在植物响应多种非生物胁迫过程中具有重要作用。基于表达谱测序数据我们分析了抗旱谷子品种(GG)和干旱敏感品种(JF)在PEG胁迫0.5 h前后LEA基因的表达变化,发现谷子LEA_1家族的Seita.6G114000基因在抗旱性不同的谷子品种中胁迫响应模式不同;利用同源序列比对和保守结构域分析我们系统鉴定得到5个谷子LEA_1家族蛋白(PF03760),该家族基因序列短,启动子区皆含多个ABRE元件,蛋白质N端序列高度保守;通过分析NCBI数据库中谷子转录组测序数据(SRA062640),发现谷子LEA_1家族基因在‘豫谷1号’中PEG胁迫7 h前后转录本丰度变化显著,表明LEA_1家族蛋白在谷子应对干旱胁迫过程中发挥重要作用。上述结果为深入研究LEA_1基因在逆境应答中的功能提供参考。

Late embryogenesis abundant（LEA） proteins have important biological function in plant responses to different abiotic stresses. The expression changes of LEA gene in drought resistant millet varieties（GG） and drought sensitive varieties（JF） 0.5 h before and after PEG stress were analyzed based on the expression spectrum sequencing data. It was found that the Seita.6 G114000 gene of the millet LEA_1 family was different in the stress response patterns of the millet varieties with different drought resistance. Further more, 5 LEA_1 family proteins（PF03760） were identified by homologous sequence alignment and conservative domain analysis. The gene sequence of the family was short, the promoter region contained multiple ABRE elements, and the N terminal sequence of the protein was highly conservative. By analyzing the sequence data of the NCBI transcriptional group（SRA062640）, it was found that the change of transcriptional abundances of the LEA_1 family gene in YG01 was significant before and after the PEG stress of 7 h, indicating that the LEA_1 family protein played an important role in the drought stress process. This study could provide reference for further study on the function of LEA_1 genes in stress response.