华北紫丁香黄烷酮3-羟化酶基因克隆及表达分析

Cloning and Expression Analysis of Flavanone 3-hydroxylase Gene from Syringa oblata Lindl.

黄烷酮3-羟化酶(flavanone 3-hydroxylase,F3H)作为黄酮类化合物合成早期的一个关键酶,在花色素合成途径中起重要作用。本研究依托华北紫丁香花序转录组高通量测序数据,结合RACE技术克隆出SoF3H基因,并对SoF3H基因进行生物信息学分析,同时采用实时荧光定量PCR技术分析了该基因的组织表达模式。结果表明,SoF3H基因的c DNA序列全长为1 101 bp,编码366个氨基酸残基;该基因的g DNA序列无内含子;SoF3H基因的组织特异性表达分析表明该基因在花中表达量最大,且在花蕾期表达量最高。本研究将为深入研究华北紫丁香黄烷酮3-羟化酶(SoF3H)在花色素代谢途径中的表达特性提供参考依据。

Flavanone 3-hydroxylase（F3H）, a key enzyme in the early synthesis of flavonoids, plays an important role in the pathway of anthocyanin biosynthesis. Based on the high-throughput sequencing data of transcriptome from inflorescence of S. oblata, SoF3H gene was cloned with the help of RACE technology. Then, the bioinformatics analysis of SoF3H gene was carried out, and the tissue expression pattern of this gene was analyzed by Real-time quantitative PCR. The results showed that the cDNA sequence of SoF3H gene was 1 101 bp in length and it encoded 366 amino acid residues. However, the g DNA sequence of the gene did not contain introns. Moreover, the tissue specific expression analysis of SoF3H gene indicated that the expression content of SoF3H was the largest in the flower, and it reached the maximum at the bud stage. This study would provide basic information for studying on the expression characteristics of SoF3H in flower pigments metabolic pathway in S. oblata.