烟草杂交组合Florda301×GDH94的杂种优势表现和cDNA-SRAP分析

Performance of Flue-cured Tobacco Heterosis of Agronomic Characters and cDNA-SRAP Analysis of Hybrid Combination Florda301×GDH94

为了解烟草杂交组合Florda301×GDH94的杂种优势表现及其形成原因,我们分析了该组合的6个农艺性状的杂种优势表现,并利用主成分分析法对各农艺性状的贡献进行了分析;利用6对SRAP引物,对Florda301×GDH94及其亲本进行c DNA-SRAP差异表达分析,并对差异表达片段进行克隆测序分析。结果表明:超高亲优势表现为株高>叶数>叶厚>叶长>叶宽>叶面积;中亲优势表现为株高>叶数>叶面积>叶长>叶宽>叶厚。叶面积和叶长、叶宽,叶长与叶宽呈极显著相关。主成分分析将6个农艺性状简化为3个主成分,即叶产量因子、长势因子、叶数因子,提供的信息占全部信息量的95.99%。6对SRAP引物在Florda301×GDH94及其亲本中共扩增出66条带,其中差异条带32条,占48.48%;差异表达类型分为UNF1、ABF1、UNP1、UNP2、DMP、DMP2六种,分别占:19.20%、8.04%、4.60%、5.75%、2.30%、6.90%。从杂种特异表达片段中分离的两条片段分别与GDP-甘露糖4,6-脱水酶和蔗糖6-果糖基转移酶同源。本研究结果为烟草农艺性状杂种优势研究提供基础理论参考。

In order to understand the performance and cause of formation of flue-cured tobacco heterosis of agronomic characters of hybrid combination Florda301 ×GDH94, We analyzed the performance and contribution by using the method of principal components of 6 agronomic characters, and c DNA-SRAP analysis of gene differential expression by using 6 pairs of SRAP primer sequences and cloning sequencing of differential expression segments after recycling. Results showed that the performance of flue-cured tobacco heterosis,over-parent heterosis presented height leaf number thickness length width leaf area and mid-parent heterosis presented heightleaf numberleaf arealengthwidththickness. Leaf area with length and length with width had the significant correlation. Principal component analysis has put 6 agronomic characters into 3 principal component as production factor, growth factor, and leaf number factor, which provided 95.99% information. There were 32 different segments, 48.48%, in 66 amplified stripes, among which gene differential expression types included UNF1, ABF1, UNP1, UNP2, DMP, and DMP2 respectively which accounted for 19.20%, 8.04%, 4.60%,5.75%, 2.30%, and 6.90%. Two fragments isolated from the specific expressed fragments of the hybrid were homologous to GDP-mannose 4,6-dehydration enzyme and Sucrose 6-transferase. The results of this study might provide theoretical reference to agronomic characters and heterosis research of tobacco.