腺嘌呤功能化石墨烯修饰电极用于地高辛血药浓度检测

Adenine-functionalized Graphene Modified Screen-printed Electrode for Detection of Plasma Concentration of Digoxin

目的:通过简单环保的合成方法制备出腺嘌呤功能化的海绵状石墨烯(FSG),并修饰于丝网印刷电极,作为一种检测地高辛(DG)血药浓度的电分析传感器。方法:合成的石墨烯材料采用扫描电子显微镜进行形态表征,采用循环伏安法检测修饰电极的电化学性能并进行优选,采用方波伏安扫描法测定修饰电极的标准曲线、特异性以及重现性,最后将修饰电极应用于实际样品中DG的检测,并与荧光偏振免疫测定法(FPIA)检测结果比较。结果:FSG修饰电极较其他修饰电极具有更优异的电化学性能,对DG表现出良好的电化学响应,其线性响应范围为0.01~100 ng·m L^(-1),检测限为5.76×10^(-3)ng·m L^(-1)。对DG的检测不受药物制剂和血浆中其他物质的影响,且能准确测出血浆样品中DG的含量,与FPIA法测定结果无显著性差异。结论:基于FSG的电化学传感器可用于临床样品和药物制剂中DG的快速检测。

Objective： Adenine-functional sponge graphene（FSG） was prepared by a simple and environmentally-friendly synthetic method and a screen-printed electrode was modified as an electroanalytical sensor for detecting plasma concentration of digoxin（DG）. Methods： The synthesized graphene materials were characterized by scanning electron microscopy. The electrochemical properties of the graphene modified electrodes were tested by cyclic voltammetry. The standard curves, specificities and reproducibility of the electrodes were determined by square wave voltammetry. Finally, the electrodes were applied to the detection of DG in actual samples and the results were compared with those of fluorescence polarization immunoassay（FPIA）. Results： The FSG modified electrodes had better electrochemical performance than other mofified electrodes and showed good electrochemical response to DG with a linear response range of 0.01--100 ng·mL^-1 and a detection limit of 5.76×10^-3 ng·mL^-1. The detection of DG was not affected by the other substances of pharmaceutical preparations and plasma. The content of DG in plasma samples could be accurately detected by FSG modified electrode, which had no significant difference from the results of FPIA. Conclusion： The FSG-based electrochemical sensor can be used for the rapid detection of DG in clinical samples and pharmaceutical preparations.