甘草甜素镁对小鼠辐射损伤的防护作用

Protective effect of glycyrrhizin magnesium (Gly-Mg) on radiation injury in mice

考察甘草甜素镁(Glycyrrhizin magnesium,Gly-Mg)的抗氧化活性及其对DNA损伤的防护作用。将小鼠分为空白对照组(生理盐水)、照射对照组(抗氧化实验:生理盐水+6.0 Gy;彗星实验:生理盐水+7.2 Gy)和照射给药组(抗氧化实验:50 mg/kg Gly-Mg+6.0 Gy、75 mg/kg Gly-Mg+6.0 Gy;彗星实验:50 mg/kg Gly-Mg+7.2 Gy、75 mg/kg Gly-Mg+7.2 Gy)。采用抗氧化试剂盒检测小鼠血清、肝组织和肺组织中超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)的活性和谷胱甘肽(Glutathione,GSH)、脂质过氧化物丙二醛(Methylene dioxyamphetamine,MDA)的含量。结果表明:Gly-Mg能够提高小鼠血清和组织中SOD、CAT的活性;增加抗氧化低分子清除剂GSH含量,并降低MDA的含量。彗星实验结果表明,与照射对照组相比,照射给药组小鼠的彗星尾长、彗星尾矩、Olive尾矩、尾部DNA含量和尾部DNA含量百分比均显著降低(p<0.01)。实验结果提示,目标化合物Gly-Mg对辐照导致的氧化损伤有一定的防护作用,而对小鼠外周血淋巴细胞的DNA损伤则具有显著的防护作用。

This study utilized antioxidant and basic single cell gel electrophoresis experiments to assess the effect of glycyrrhizin magnesium（Gly-Mg） on oxidative and DNA damage. ICR（Institute of Cancer Research） mice were divided into a control group（normal saline）, irradiation control group（antioxidant experiment： normal saline＋6.0 Gy; comet test： normal saline＋7.2 Gy）, and Gly-Mg group（antioxidant experiment： 50 mg/kg＋6.0 Gy, 75 mg/kg＋6.0 Gy; comet test： 50 mg/kg＋7.2 Gy, 75 mg/kg＋7.2 Gy）. The activity of superoxide dismutase（SOD） and catalase（CAT）, and the content of glutathione（GSH） and methylene dioxyamphetamine（MDA） in the serum, liver, and lung tissues of mice were detected using antioxidant kits. Results of the oxidative damage protection tests suggested that Gly-Mg can improve the activities of SOD and CAT, increase the content of GSH, and reduce the content of MDA in mouse serum and tissues. Meanwhile, results of the comet test showed that Gly-Mg groups had a significant protective effect on radiation-induced DNA damage in bone marrow compared with that in the irradiation control group（p〈0.01）. In conclusion, Gly-Mg has protective effect against antioxidant and DNA damage.