恶性疟红细胞期葡萄糖转运关键蛋白的识别

Recognition of Key Proteins for Glucose Permeation During Plasmodium falciparum Blood Stage

恶性疟原虫(Plasmodium falciparum)感染人体后经历肝细胞期和红细胞期2个阶段.红细胞期是恶性疟原虫在人体内发育增殖和产生临床症状的主要阶段,其能量代谢完全依赖于吸收血液中的葡萄糖.葡萄糖首先通过宿主红细胞膜(erythrocyte plasma membrane,EPM)上的葡萄糖转运蛋白1(Glucose transporter 1,GLUT1)进入红细胞胞浆,再由疟原虫质膜(parasite plasma membrane,PPM)上的己糖转运蛋白(P.falciparum hexose transporter,Pf HT)完成疟原虫对葡萄糖的吸收.针对恶性疟原虫红细胞期葡萄糖转运途径中的关键蛋白进行序列分析,合成相应多肽,并获得了特异性识别GLUT1和Pf HT蛋白的抗体,为葡萄糖转运蛋白在恶性疟原虫感染中的机制研究,及抗疟药物治疗靶点的探索提供了必要条件.

Host infection of Plasmodium parasites undergoes liver stage and blood stage, the latter of which is the main stage for parasitic proliferation and clinical symptoms development in human. The energy metabolism during this stage relies completely on glucose from the host blood stream. Glucose first entersred blood cells（RBCs） through glucose transporter 1（GLUT1） on the erythrocyte plasma membrane, and is further taken up by the parasites by Plasmodium hexose transporter（HT） on the parasite plasma membrane. Here, we analyzed sequences of human GLUT1 and P. falciparum HT（Pf HT）, identified antigenic peptides, and generated antibodies against these proteins. Western blot analysis revealed that the antibodies can specific recognize endogenous GLUT1 and Pf HT. GLUT1 antibodies obtained here are more efficient than commercial GLUT1 antibodies. These results provide necessary tools for investigating glucose permeation pathway during Plasmodium falciparum blood stage infection.