肾炎康复片对单侧输尿管梗阻大鼠肾间质纤维化的保护作用

Protection of Shenyankangfu tablet on renal interstitial fibrosis in unilateral ureteral occlusion rats

目的:探讨肾炎康复片对单侧输尿管梗阻(unilateral ureteral occlusion,UUO)大鼠肾间质纤维化(renal interstitial fibrosis,RIF)的保护作用。方法:雌性SD大鼠24只,随机分为假手术组(sham组)、UUO组和肾炎康复片组(SYKF组),每组各8只。SYKF组给予肾炎康复片600 mg/(kg·d)灌胃,sham组和UUO组予同体积的生理盐水灌胃。14 d后处死各组大鼠,取左肾组织作HE染色和Masson染色观察肾组织学变化;采用实时定量荧光聚合酶链反应(real-time polymerase chain reaction,real-time PCR)的方法检测肾α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)和E-钙黏蛋白(E-cadherin)的mRNA水平;用Western印迹法检测肾α-SMA和E-cadherin蛋白的表达情况。结果:UUO组肾组织出现明显RIF改变,SYKF组RIF程度较UUO组明显减轻;UUO组大鼠肾α-SMA mRNA和蛋白表达水平较sham组明显升高(P<0.01);而E-cadherin mRNA和蛋白的表达水平较sham组明显下调(P<0.01)。与UUO组相比,SYKF组肾α-SMA mRNA和蛋白表达水平明显下调(P<0.05),而E-cadherin mRNA和蛋白表达水平显著升高(P<0.05)。结论:肾炎康复片对UUO大鼠RIF起一定的保护作用,其机制可能与肾小管上皮细胞转分化有关。

Objective： To investigate the protective effects of Shenyankangfu tablet on renal interstitial fibrosis in unilateral ureteral obstruction（UUO） rats. Methods： Twenty-four SD rats were randomly divided into three groups, including the sham group, the UUO group and the SYKF group（n=8）. Rates of the SYKF group were given a gavage with Shenyankangfu tablet at a dose 600 mg/（kg·d）; the sham and the UUO groups rats were treated with normal saline of the same volume. After executing all the rats at 14 d, changes in renal pathology were observed by HE and Masson staining, and the expressions of α-smooth muscle actin（α-SMA）, E-cadherin mRNA and protein were measured by real-time polymerase chain reaction（real-time PCR） and Western blot. Results： Renal interstitial fibrosis was observedin the UUO group. However, renal interstitial fibrosis was ameliorated significantly in the SYKF group. The expressions of α-SMA mRNA and protein were significantly increased in the UUO group（P〈0.01）, but the opposite expressions of E-cadherin mRNA and protein（P〈0.01） compared with those in the sham group. The expressions of α-SMA mRNA and protein were significantly decreased（P〈0.05）, while E-cadherin mRNA and protein were increased（P〈0.05） in the SYKF group compared with UUO group. Conclusion： Shenyankangfu tablet maybe have protective effects on renal interstitial fibrosis potentially associated with transdifferentiation of renal tubular epithelial cells.