微小RNA-9与Wnt/β连环素在星形细胞肿瘤的表达及其临床意义

Expression of microRNA-9 and Wnt/β-catenin in astrocyte tumors and its clinical significance

目的:探讨不同级别的星形细胞肿瘤微小RNA-9(microRNA-9,miR-9)和Wnt/β连环素(β-catenin)mRNA及其异常表达与临床病理参数的关系。方法:收集2012至2017年新疆医科大学第一附属医院经手术切除并病理诊断的胶质细胞肿瘤102例,包括弥漫型星形细胞瘤(Ⅱ级)36例、间变性星形细胞瘤(Ⅲ级)36例、胶质母细胞瘤(Ⅳ级)30例。采用RT-PCR检测β-catenin mRNA的异常表达,用χ~2检验、回归分析、SPSS生存分析方法分析其异常表达与星形细胞肿瘤的临床病理参数及其预后的关系。结果:在Ⅱ级、Ⅲ级、Ⅳ级星形细胞肿瘤中,分别有27,30,30例miR-9呈高表达;分别有24,33,30例β-catenin呈高表达。随胶质细胞肿瘤级别的增高,miR-9和β-catenin mRNA表达水平增高(P<0.05)。miR-9 mRNA水平的异常表达与患者年龄、肿瘤发病部位及组织学类型差异有统计学意义(P<0.05);β-catenin mRNA水平的异常表达与组织学类型显著相关(P<0.05)。星形细胞肿瘤患者预后与miR-9和β-catenin mRNA水平的异常表达显著相关(P<0.05)。结论:miR-9,β-catenin mRNA有望成为星形细胞肿瘤的相关预后指标。

Objective： To explore the relationship between the abnormal expression of microRNA-9（miR-9） and Wnt/β-catenin mRNA in different levels of astrocytoma and its clinical and pathological parameters. Methods： We collected 102 cases glial cell tumor combined by pathological diagnosis of including diffuse astrocytoma 36 cases（grade Ⅱ）, anaplastic astrocytoma 36 cases（grade Ⅲ）, glioblastoma 30 cases（grade Ⅳ） during 2012—2017 in First Affiliated Hospital, Xinjiang Medical University. RT-PCR was used to dectect the β-catenin abnormal mRNAexpression. The relationship between the abnormal expression and the clinical and pathological parameters and prognosis of glial cell tumor were analyzed by χ~2 test, regression analysis, survival analysis methods with SPSS.Results： MiR-9 showed high expression in 27, 30 and 30 cases in grade Ⅱ–Ⅳ astrocytic tumor, respectively. There were 24 cases, 33 cases, and 30 cases showed high expression of β-catenin. With the increase of the tumor level of glial cells, the abnormal expression level of miR-9, β-catenin mRNA was increased（P〈0.05）. The abnormal expression level of miR-9 mRNA was correlated with the age of the patient, the site of the tumor and histological type（P〈0.05）. The abnormal expression of β-catenin mRNA level was correlated with histological type（P〈0.05）.The prognosis of astrocytic tumor patients was correlated with the abnormal expression of miR-9 mRNA andβ-catenin mRNA levels（P〈0.05）. Conclusion： MiR-9 and β-catenin mRNA are expected to be the prognostic indicators of astrocyte tumor.