酶联免疫吸附筛法测定大米和大麦中黄曲霉毒素B1的含量

Determination of aflatoxin B1 in brewing grands by ELISA

建立了大米和大麦中黄曲霉毒素B，的酶联免疫吸附测定方法，样品经25mL甲醇-水（50：50，v／v）提取后，用酶联免疫法直接测定。结果表明，黄曲霉毒素B1在1．0μg／kg-20．0μg／kg范围内拟合度良好，相关系数为0．9999，平均加标回收率为97％～113％，相对标准偏差为1．9％～4．8％。对实际样品进行检测时发现，在不良环境下储存的大麦样品中AFTB，的含量会增加。

An enzyme-linked immunosorbent assay （ELISA） for the determination of aflatoxin B1 in rice and barley was established. The samples were extracted by 25mL methanol water （50：50, v/v）. A good linear relationship （r2=0.9999） was found in the concentration range of 1.0-20.0μg/kg. The recovery and the relative standard deviation of the sample are 97%-113%, and 1.9%-4.8%, respectively. The content of aflatoxin B t will increase in 30 humidity and poor ventilation environment.