HPLC同时测定新疆不同产地新塔花中3种有效成分的含量

Determination of 3 components in Xinjiang Ziziphora bungeana by HPLC

为更加全面的反映维吾尔族药材新塔花的内在质量,对新塔花中的多种成分进行HPLC同步测定,比较不同产地新塔花中咖啡酸、迷迭香酸、蒙花苷的含量。样品制备采用回流提取,选用ZORBAX SB-C18色谱柱（4.6 mm×250 mm,5.0μm）;流动相为甲醇（A）-0.2%冰醋酸溶液（B）,梯度洗脱,检测波长340 nm,柱温30℃,流速1.0 m L·min-1。咖啡酸、迷迭香酸、蒙花苷分别在240 mg·L-1（r=0.999 9）,360 mg·L-1（r=1）,7140 mg·L-1（r=0.999 9）呈良好的线性关系,平均回收率分别为100.9%（RS 1.3%）,98.25%（RSD 2.0%）,101.2%（RSD 1.5%）。测得13批新塔花中咖啡酸质量分数在0.081.07mg·g-1、迷迭香酸质量分数在1.233.59 mg·g-1、蒙花苷质量分数在2.0810.90 mg·g-1。该方法结果准确、重复性好,可用于测定新塔花药材中咖啡酸、迷迭香酸、蒙花苷的含量;3种成分在不同批次的新塔花药材中的含量差异显著,其内在质量差异较为明显。

This study aimed to develop an HPLC method for simultaneous determination of the 3 components in Ziziphora bungeana.The optimum HPLC condition was as follows： ZORBAX SB-C18 column（ 4. 6 mm × 250 mm,5. 0 μm） with gradient elution of methanol（ A）-0. 2% glacial acetic acid（ B）,detection wavelength 340 nm,column temperature 30 ℃,flow rate 1 m L·min-1. There were good linearity between peak areas and injection quantity of caffeic acid,rosmarinic acid,and linarin in the range of 2-40 mg·L-1（ r =0. 999 9）,3-60 mg·L-1（ r = 1）,7-140 mg·L-1（ r = 0. 999 9）,respectively. The average recoveries were 100. 9%（ RSD 1. 3%）,98. 25%（ RSD 2. 0%）,and 98. 73%（ RSD 1. 5%）,respectively. The HPLC method was stable and accurate,which could be used to detect caffeic acid,rosmarinic acid,and linarin in Ziziphora bungeana.