HPLC-FT-MS鉴定荜茇总生物碱在大鼠粪便及尿液中的代谢产物

Identify metabolites of Piper longum alkaloids in feces and urine of rat by HPLC-FT-MS

研究荜茇总生物碱中主要成分在正常大鼠粪便及尿液的代谢产物,推测其代谢途径。通过灌胃给予大鼠荜茇总生物碱后,收集粪便及尿液并进行预处理,采用高效液相色谱与傅里叶变换离子回旋共振质谱仪联用技术（HPLC-FT-MS）进行检测,根据高分辨质谱数据,并与文献对照,推测代谢产物的结构。在大鼠的粪便及尿液中检测到胡椒碱、胡椒新碱、荜茇明宁碱、二氢荜茇明宁碱、墙草碱及其13个代谢产物,进而提示荜茇总生物碱中的主要成分可能在大鼠体内通过Ⅰ相、Ⅱ相代谢反应产生代谢产物,并经粪便及尿液排出体外。为阐明荜茇总生物碱在体内发挥药效的物质基础和作用机制奠定基础。

The metabolites were detected in feces and urine of rats orally administrated alkaloids of Piper longum by using high performance liquid chromatography coupled with a Fourier Transform ion cyclotron resonance mass spectrometer（ HPLC-FT-MS）. According to the mass spectrometric data and reported literature,the structures of metabolites were identified. Several metabolites were analyzed and belonged to piperine,piperanine,piperlonguminine,Δα,β-dihydropiperlonguminine and pellitorine,respectively. The metabolites of alkaloids from P. longum alkaloids were produced through I phase and Ⅱ phase metabolism reaction,and were excreted with urination and defecation. The approach provided a rapid method for characterizing the metabolites of P. longum alkaloids and gave the truly active structures and the action mechanism of their neuroprotective effects.