甘草内生真菌的分离及抑菌活性研究

目的：分离和纯化甘草肉生真菌，并检测其次级代谢产物抑菌活性。方法：采用组织块分离法从根中分离内生真菌，采用滤纸片法去评价甘草内生真菌代谢产物抑菌活性，结果：从甘草根得到15株内生真菌，获得乙酸乙酯、正丁醇、甲醇部位提取物其45个，其中43个样品显示一定抑菌活性，占样品总数95．6％，其中R108（EAE）、R108（NBE）、R109（EAE）、R109（NBE）、R109（MME）这5个样品均对绿脓杆菌（Pseudomonas aeruqinosa）显示较强抑菌活性。最小抑菌浓度分别为1．25mg／mL、0．625mg／mL、2．5mg／mL、0．3125mg／mL、2．5mg／mL，结论：甘草内生真菌发酵产物具有一定抑菌活性，抑菌范围较广，具有一定研究价值。

Objective： Endophytic fungi were isolated and purified from Glycyrrhiza uralensis and the bacteriostatic activities of their secondary metabolites were examined. Methods： Endophytic fungi were isolated from the roots by tissue isolation, and evaluate its bacteriostatic activities through filter paper method. Results： The number of endophytic fungi which we obtained from glycyrrhiza root is 15, and the number of extract from ethyl acetate, N-butanol and carbinol is 45. Among them, we found there were 43 samples, which had bacteriostatic activities, and the total number of sample is 95,6%. Furthermore, R108 （ EAE ） , R108 （ NBE ） , R109 （ EAE ） , R109 （ NBE ） and R109 （ MME ） had the bacteriostatic activities to Pseudomonas aeruginosa, and the minimum inhibitory concentration was l.25mg/ml, 0.625mg/ ml, 2.5mg/ml, 0.3125mg/ml, 2.5mg/ml. Conclusion： The fermented products of endophytic fungi have a widespread value of research, which have a certain bacteriostasis activity, and a wide bactenostas^s range.