无创检测口腔白斑及鳞癌中人乳头瘤病毒及分型

Non-invasive human papilloma virus genotypes detection in oral leukoplakia and squamous cell carcinomas

目的探讨口腔白斑(oral leukoplakia,OLK)和口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中人乳头瘤病毒(human papillomavirus,HPV)及其分型和临床意义。方法采用无创方法收集30例正常人、103例OLK、30例OSCC的口腔黏膜脱落细胞,采用聚合酶链反应(Polymerase chain reaction,PCR)结合导流杂交技术进行37种HPV基因型别的检测,对受检者进行相关资料分析。结果正常人检出HPV阳性1例,检出率为3.33%(1/30);OLK检出HPV阳性5例,检出率为4.85%(5/103);OSCC检出HPV阳性1例,检出率为3.33%(1/30);各组间差异无统计学意义(χ~2=0.22,P=0.90>0.05)。其中OLK中4例为HPV单一型阳性,1例为HPV三重型别阳性。结论 PCR导流杂交检测技术能无创检测口腔HPV分型情况,可应用于临床。HPV在OLK、OSCC中检出率较低,可能与OLK、OSCC的发病关联性不强。

Objective To detect human papilloma virus（ HPV） genotypes in oral leukoplakia（ OLK） and oral squamous cell carcinoma（ OSCC） and its clinical significance. Methods Polymerase chain reaction（ PCR） and diversion hybridization technology were utilized to detect 37 kinds of HPV genotypes in oral mucosa exfoliated cells from 30 cases of normal mucosa,103 cases of OLK and 30 cases of OSCC,respectively. Clinicopathological characteristics of all subjects were also analyzed. Results In normal oral mucosa,HPV detection rate was 3.33%（ 1/30）. In OLK,HPV detection rate was 4.85%（ 5/103）. In OSCC,HPV detection rate was 3.33%（ 1/30）. There was no statistically significant difference among the groups（ chi-square= 0.22,P= 0.90〉0.05）. 4 OLK cases were detected with 1 kind of HPV,and 1 case was detected with 3 kinds of HPV. Conclusions Non-invasive sampling with PCR and diversion hybridization technology can be applied to clinically detect HPV genotypes in oral mucosa. The detection rates of HPV in OLK and OSCC are low,and HPV may not be the main pathogenesis factor.