刚毛藻3种培养方式比较

Comparison of three culture methods for Cladophora oligoclona

刚毛藻野外生长旺盛,但是室内培养生长效果不好.为促进刚毛藻生态学、水环境治理特性研究及开发利用,以寡枝刚毛藻（Cladophora oligoclona）为对象,分析3种培养方式（静置、通气、水体流动＋通气）对刚毛藻生物量、形态和光合活性变化的影响,以建立和评估刚毛藻室内培养的方法.结果显示,水体流动＋通气培养方式能有效促进刚毛藻生物量的积累;刚毛藻在3种培养方式下培养,形态变化不同,在水体流动＋通气培养方式下单个细胞的长度变长、直径变短,从而表现出主枝细胞长度、分枝细胞长度、株高、分枝总长显著高于静置和通气培养方式培养的刚毛藻（P〈0.05）;刚毛藻在水体流动＋通气培养方式下显示出更高的光合活性.综上表明,水体流动＋通气培养方式更适合刚毛藻的室内培养,该方法可为刚毛藻室内进一步的大量培养提供技术参考.

The growth of Cladophora spp. is vigorous in the field, but not good under indoor culture conditions. In order to promote the study on ecology, water environment treatment characteristics, and the development and utilization of Cladophora, the effects of three different culture methods（standing, ventilating, water flowing ＋ ventilating） on biomass, morphology, and photosynthetic activity of C. oligoclona were compared in order to find a suitable method for its indoor culture. The results showed that the culture method of water flowing ＋ ventilating could effectively promote C. oligoclona biomass accumulation; the morphologies in the three culture methods were different. C. oligoclona with increased single cell length and reduced cell diameter was found in the culture method involving water flowing ＋ ventilating; in this method, the length of the main branch and branch of the cell, plant height, and branch length of C. oligoclona were significantly higher than that in the standing and ventilating culture methods（P〈0.05）. C. oligoclona cultured in the water flowing ＋ ventilating method also showed a higher photosynthetic activity. In conclusion, among the three culture methods, the water flowing ＋ ventilating culture method is the most suitable for the indoor culturing of C. oligoclona; this method can also provide a technical reference for the further indoor mass culture of C. oligoclona.