miR-1301在甲状腺乳头状癌中的表达变化及其临床意义

Expression of miR-1301 in thyroid papillary carcinoma and its clinical significance

目的探讨miR-1301、miR-1976在甲状腺乳头状癌(PTC)中的表达情况及其临床意义。方法收集2015年6月~2016年6月在我院甲状腺外科行甲状腺手术的65例患者的甲状腺肿瘤组织、肿瘤旁正常甲状腺组织,其中良性35例,乳头状癌组织30例。在TCGA中选取PTC可能表达异常的miR-1301、miR-1976,通过qRT-PCR验证,并分析其临床相关性;通过在线数据库Targetscan、GEPIA预测miR-1301的靶基因。结果 miR-1301在PTC癌组织中表达降低(P<0.01),miR-1976在PTC癌组织中表达无明显变化(P>0.05);临床分析表明,miR-1301表达变化与PTC的病理分级"T"、"N"相关(P=0.005,P=0.045);ROC曲线提示miR-1301对PTC具有诊断价值,AUC=0.774,敏感性50%,特异性90%。生信分析发现,miR-1301可能通过对CLDN1、TRPC5的调控,参与PTC的生长、凋亡及迁移等。miRNA-1301在PTC癌组织中表达降低,且与PTC临床病理特点相关,可能通过对其靶基因的负性调控抑制PTC的发生与进展。结论 miRNA-1301可作为PTC诊断、术前风险评估、预后分析的潜在分子标志物。

Objective To determine the expression profiles of miR-1301 in the papillary thyroid cancer tissues and analyze its clinical relevance and discuss the possibility of miR-1301 as a biomarker for PTC diagnosis.Methods From June 2015 to June 2016,65 patients of thyroid papillary carcinoma and paracancerous normal thyroid tissues were collected from thyroid surgery in our hospital,including 35 benign cases and 30 papillary carcinoma tissues.miR-1301 and miR-1976 from TCGA were validated by qRT-PCR.By online database Targetscan and GEPIA,the target genes of miR-1301 in the PTC were estimated.Results qRT-PCR performed to determine the expression profiles of miR-1301 was downregulated in the papillary thyroid cancer tissues.miR-1976 was no significant difference among paraneoplastic tissue,benign tumor tissue and papillary thyroid cancer tissues.Analyzing clinic features found miR-1301 expression levels association with PTC＇s＂T＂and＂N＂stages.ROC curve indicated miR-1301 may act as a potential diagnostic marker for PTC,and AUC was 0.774.With bioinformatic analysis,we speculated that miR-1301 may participate in PTC growth,apoptosis,migration and metastasis by targeting CLDN1 and TRPC5.In conclusion,we revealed that miR-1301 was Downregulated in the PTC tissues and we speculated that serve as a anti-oncogene by targeting CLDN1 and TRPC5.Conclusion miR-1301 may act as a biomarkers for PTC＇s diagnosis,preoperative assessment and prognostic analysis.